Genetic Variation within and among Iranian, French and Danish Rainbow Trout (Oncorhynchus mykiss) Using PCR-RFLP Analysis of Mitochondrial DNA Segments

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Shakki Rabi; RAHMANI HOSSEIN; Rahimi Ghodrat; GHOLIZADEH MOHSEN

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Journal: Journal of Fisheries Year:2017 | Volume:70 | Issue:1 Page(s): 106-116

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Title

Genetic Variation within and among Iranian, French and Danish Rainbow Trout (Oncorhynchus mykiss) Using PCR-RFLP Analysis of Mitochondrial DNA Segments

Author(s)

Shakki Rabi | RAHMANI HOSSEIN | Rahimi Ghodrat | GHOLIZADEH MOHSEN | Issue Writer Certificate

Keywords

mtDNAQ2

PCR-RFLPQ2

Rainbow troutQ1

Genetic diversityQ1

Abstract

The objective of this study was to investigate the Genetic diversity within and between Iranian, French and Danish Rainbow trout, Oncorhynchus mykiss. Mitochondrial DNA (mtDNA) of 135 samples (45per each breed), isolated by phenol-chloroform extraction method, was studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Fragments that included 986 bp of D-loop and 974 bp of Cyt b of mtDNA were amplified and investigated using different restriction enzymes. None of samples showed variation for restriction enzymes in D-loop. Using restriction enzymes, on gel electrophoresis, four different haplotypes were detected in Cyt b among which two breed specific haplotypes were observed in Danish population. AABBAB haplotypes had the most frequency with 61 events. UPGMA dendrogram based on cytochrome b gene showed that the Iranian population make cluster with French population rather than the Danish population. The genetic distance was 0. 0008 between Iranian and French populations and 0. 198 between both Iranian and Danish and French and Danish populations.

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APA: Copy

Shakki, Rabi, RAHMANI, HOSSEIN, Rahimi, Ghodrat, & GHOLIZADEH, MOHSEN. (2017). Genetic Variation within and among Iranian, French and Danish Rainbow Trout (Oncorhynchus mykiss) Using PCR-RFLP Analysis of Mitochondrial DNA Segments. JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES), 70(1 ), 106-116. SID. https://sid.ir/paper/162255/en

Vancouver: Copy

Shakki Rabi, RAHMANI HOSSEIN, Rahimi Ghodrat, GHOLIZADEH MOHSEN. Genetic Variation within and among Iranian, French and Danish Rainbow Trout (Oncorhynchus mykiss) Using PCR-RFLP Analysis of Mitochondrial DNA Segments. JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES)[Internet]. 2017;70(1 ):106-116. Available from: https://sid.ir/paper/162255/en

IEEE: Copy

Rabi Shakki, HOSSEIN RAHMANI, Ghodrat Rahimi, and MOHSEN GHOLIZADEH, “Genetic Variation within and among Iranian, French and Danish Rainbow Trout (Oncorhynchus mykiss) Using PCR-RFLP Analysis of Mitochondrial DNA Segments,” JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES), vol. 70, no. 1 , pp. 106–116, 2017, [Online]. Available: https://sid.ir/paper/162255/en

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