IDENTIFICATION OF THE PREVALENT POTYVIRUS ON MAIZE IN CORN FIELDS OF TEHRAN PROVINCE AND A STUDY ON SOME OF ITS PROPERTIES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOHAMMADI M.R.; KOOHI HABIBI M.; MOSSAHEBI G.H.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Plant Protection Science Year:2009 | Volume:40 | Issue:1 Page(s): 43-53

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

802

Download:

Cites:

Information Journal Paper

Title

IDENTIFICATION OF THE PREVALENT POTYVIRUS ON MAIZE IN CORN FIELDS OF TEHRAN PROVINCE AND A STUDY ON SOME OF ITS PROPERTIES

Author(s)

MOHAMMADI M.R. | KOOHI HABIBI M. | MOSSAHEBI G.H. | Issue Writer Certificate

Keywords

POTYVIRUSQ2

MAIZEQ2

SUGARCANE MOSAIC VIRUSQ2

ELISAQ2

TEHRAN PROVINCEQ3

Abstract

Two hundred and thirty one leaf samples of virus infected and mosaic and as well dwarf mosaic symptoms showing MAIZE plants from various corn fields in TEHRAN PROVINCE were collected during the growing season 2003. By performing serological tests of DAS-ELISA, DIBA and TPIA, using SCMV, MDMV, SrMV and JGMV antisera, only SCMV was detected in samples, with all samples reacting strongly to the SCMV antiserum. SCMV was determined as the main and prevalent POTYVIRUS on MAIZE in TEHRAN PROVINCE. The virus was transmitted to sweet corn and sorghum plants using 0.1 M potassium phosphate buffer (pH 7) containing 2% Polyvinyl Pyrrolidon (PVP) for mechanical inoculation. For propagation and maintenance in greenhouse, the virus was propagated on sweet corn cv. Pars 403 and on grain sorghum cv. Kimia. The host range study with selected isolates of SCMV showed that the virus isolates were not transmitted by mechanical inoculation on Avena sativa, Panicum miliaceum, Setaria italica, Pennisetum americanum, Hordeum vulgare and Triticum aestivum. Purification of isolates was done using a minipurification method. UV absorbance spectra were obtained for purified viruses a ratio of A260/280=1.2 being calculated. Electron microscopic study using ISEM and decoration method with SCMV antiserum revealed filamentous flexuous particles of SCMV. In SDS-PAGE and western blotting tests on infected samples and purified isolates, the molecular weight of the virus coat protein was approximately 37-38 KDa. A difference among the CP of various SCMV isolates however, was not detected. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was done using SCMV F3 and SCMV R3 primers. Where amplified fragments were found of approximately 900 bp in size as expected. The study indicated that SCMV is the prevalent POTYVIRUS and the main causal agent of mosaic and dwarf mosaic on MAIZE plants in the province.

Cites

No record.

References

No record.

Cite

APA: Copy

MOHAMMADI, M.R., KOOHI HABIBI, M., & MOSSAHEBI, G.H.. (2009). IDENTIFICATION OF THE PREVALENT POTYVIRUS ON MAIZE IN CORN FIELDS OF TEHRAN PROVINCE AND A STUDY ON SOME OF ITS PROPERTIES. IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 40(1), 43-53. SID. https://sid.ir/paper/171527/en

Vancouver: Copy

MOHAMMADI M.R., KOOHI HABIBI M., MOSSAHEBI G.H.. IDENTIFICATION OF THE PREVALENT POTYVIRUS ON MAIZE IN CORN FIELDS OF TEHRAN PROVINCE AND A STUDY ON SOME OF ITS PROPERTIES. IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2009;40(1):43-53. Available from: https://sid.ir/paper/171527/en

IEEE: Copy

M.R. MOHAMMADI, M. KOOHI HABIBI, and G.H. MOSSAHEBI, “IDENTIFICATION OF THE PREVALENT POTYVIRUS ON MAIZE IN CORN FIELDS OF TEHRAN PROVINCE AND A STUDY ON SOME OF ITS PROPERTIES,” IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 40, no. 1, pp. 43–53, 2009, [Online]. Available: https://sid.ir/paper/171527/en

Related Journal Papers