The characterization of three isolates of Potato virus Y from khuzestan province according to the coat protein

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Zabihi Seyed Sajjad; Hosseini Samin Sadat; HOSSEINI SEYED AHMAD

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Plant Protection Science Year:2017 | Volume:48 | Issue:1 Page(s): 97-107

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

610

Download:

Cites:

Information Journal Paper

Title

The characterization of three isolates of Potato virus Y from khuzestan province according to the coat protein

Author(s)

Zabihi Seyed Sajjad | Hosseini Samin Sadat | HOSSEINI SEYED AHMAD | Issue Writer Certificate

Keywords

Coat proteinQ1

turnipQ1

Potato virus YQ1

RT-PCRQ2

SDS-PAGEQ1

Abstract

Potato virus Y is one of the most important damaging viruses with a broad host range. In this research due to detection and isolation of Potato virus Y in turnip fields of Khuzestan province during 2014 growing season, characteristics of three selected isolates (7Tu, 9Tu and 10Tu) from Dezful, Shushtar and Shush cities were investigated. To analyze the host range of the isolates, three selected isolates were mechanically inoculated on some members of Solanaceae, Chenopodiaceae, and Amaranthaceae. All three isolates induced local and systemic symptoms on the leaves of potato, tomato, pepper, datura, and gomphrena plants, but no symptoms were observed on Chenopodium quinoa. Analysis of the PVY partially purified preparations indicated that molecular weight of two protein bands obtained by SDS-PAGE was estimated to be about 33 and 31 KDa. To determine the strain of selective isolates the specific primer pairs CF/OR, NF/NR, OF/OR, and NF/OR were used. Using OF/OR and PVY3TIM/PVNIBIP primers, two fragments with 609 and 1200 bp length respectively were amplified. The CP region in the all three isolates was amplified, cloned and sequenced. The results of all aforementioned tests showed that 7Tu, 9Tu, and 10Tu isolates are probably a member of NTN or W strain groups. According to our knowledge, this is the first report on occurrence of PVY in turnip fields.

Cites

No record.

References

No record.

Cite

APA: Copy

Zabihi, Seyed Sajjad, Hosseini, Samin Sadat, & HOSSEINI, SEYED AHMAD. (2017). The characterization of three isolates of Potato virus Y from khuzestan province according to the coat protein. IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 48(1 ), 97-107. SID. https://sid.ir/paper/171594/en

Vancouver: Copy

Zabihi Seyed Sajjad, Hosseini Samin Sadat, HOSSEINI SEYED AHMAD. The characterization of three isolates of Potato virus Y from khuzestan province according to the coat protein. IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2017;48(1 ):97-107. Available from: https://sid.ir/paper/171594/en

IEEE: Copy

Seyed Sajjad Zabihi, Samin Sadat Hosseini, and SEYED AHMAD HOSSEINI, “The characterization of three isolates of Potato virus Y from khuzestan province according to the coat protein,” IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 48, no. 1 , pp. 97–107, 2017, [Online]. Available: https://sid.ir/paper/171594/en

Related Journal Papers