BIOCHEMICAL CHARACTERIZATION OF Α- AMYLASE IN MELON LADYBIRD, EPILACHNA CHRYSOMELINA AND INHIBITORY EFFECTS OF INHIBITORS EXTRACTED FROM PLANT SEEDS ON ITS ACTIVITY

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GHANBARI NEJAD ROUHANGIZ; GHADAMYARI MOHAMMAD; HASSAN SAJEDI REZA

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Journal: Iranian Journal of Plant Protection Science Year:2015 | Volume:45 | Issue:2 Page(s): 251-263

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Title

BIOCHEMICAL CHARACTERIZATION OF Α- AMYLASE IN MELON LADYBIRD, EPILACHNA CHRYSOMELINA AND INHIBITORY EFFECTS OF INHIBITORS EXTRACTED FROM PLANT SEEDS ON ITS ACTIVITY

Author(s)

GHANBARI NEJAD ROUHANGIZ | GHADAMYARI MOHAMMAD | HASSAN SAJEDI REZA | Issue Writer Certificate

Keywords

α- AMYLASEQ2

MELON LADYBIRDQ2

BIOCHEMICAL CHARACTERIZATIONQ2

INHIBITORQ2

Abstract

Epilachna chrysomelina is one of major pests of cucurbitaceous plants. Biochemical properties of a- amylase were studied in MELON LADYBIRD. The optimal pH for a- amylase was found at 4. Also, high amylolytic activity was found at temperature 50oC. The specific activity of this enzyme in digestive system of different larval instar, foregut, midgut, hindgut and female and male adults were determined. Results showed that the highest specific a- amylase activity was detected in the digestive system of 3rd instar larvae and the enzyme activity in males and females digestive system did not show any significant difference. The enzyme specific activity in midgut was 5- and 8.3-fold higher than foregut and hindgut. The Michaelis–Menten constant (Km) of a-amylase was obtained as 0.69 mg/ml. Zymogram analysis of a- amylase showed one isoform. Also, INHIBITORy effect of INHIBITORs extracted from seeds of Lathyrus sativus, Trifolium alexandrium, Zea mays, Faba vulgaris, Lentis culinaris, Vigna unguiculata, Phaseolus vulgaris and Vigna radiate were evaluated on E. chrysomelina a- amylase activity. Among these INHIBITORs, the INHIBITORs extracted P. vulgaris and V. radiate were able to inhibit E. chrysomelina amylase.

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APA: Copy

GHANBARI NEJAD, ROUHANGIZ, GHADAMYARI, MOHAMMAD, & HASSAN SAJEDI, REZA. (2015). BIOCHEMICAL CHARACTERIZATION OF ?- AMYLASE IN MELON LADYBIRD, EPILACHNA CHRYSOMELINA AND INHIBITORY EFFECTS OF INHIBITORS EXTRACTED FROM PLANT SEEDS ON ITS ACTIVITY. IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 45(2), 251-263. SID. https://sid.ir/paper/171616/en

Vancouver: Copy

GHANBARI NEJAD ROUHANGIZ, GHADAMYARI MOHAMMAD, HASSAN SAJEDI REZA. BIOCHEMICAL CHARACTERIZATION OF ?- AMYLASE IN MELON LADYBIRD, EPILACHNA CHRYSOMELINA AND INHIBITORY EFFECTS OF INHIBITORS EXTRACTED FROM PLANT SEEDS ON ITS ACTIVITY. IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2015;45(2):251-263. Available from: https://sid.ir/paper/171616/en

IEEE: Copy

ROUHANGIZ GHANBARI NEJAD, MOHAMMAD GHADAMYARI, and REZA HASSAN SAJEDI, “BIOCHEMICAL CHARACTERIZATION OF ?- AMYLASE IN MELON LADYBIRD, EPILACHNA CHRYSOMELINA AND INHIBITORY EFFECTS OF INHIBITORS EXTRACTED FROM PLANT SEEDS ON ITS ACTIVITY,” IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 45, no. 2, pp. 251–263, 2015, [Online]. Available: https://sid.ir/paper/171616/en

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