Identification and partial characterization of the virus infecting saffron (Crocus sativus) in Iran

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Parizad Shirin; DIZADJI AKBAR; KOOHI HABIBI MINA; Mosahebi Mohammadi Gholam Hossein; KALANTARI SIAMAK; IZADPANAH FATEMEH; Garcia Arenal Fernando; WINTER STEPHAN

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Journal: Iranian Journal of Plant Protection Science Year:2017 | Volume:47 | Issue:2 Page(s): 263-275

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Title

Identification and partial characterization of the virus infecting saffron (Crocus sativus) in Iran

Author(s)

Keywords

Coat proteinQ2

ELISAQ1

PotyvirusQ2

saffron (Crocus sativus L.)Q1

Abstract

Following the random sampling of saffron (Crocus sativus L. ) leaves and corms from six provinces of Iran, during growing seasons of 2011-2015, a total of 890 leaf tissue samples were investigated by enzyme-linked immunosorbent assay (ELISA) tests. The results revealed that 641 samples showed positive reaction only with Potyvirus genus specific and exactly with Bean common mosaic virus (BCMV) specific antibodies. The molecular mass of several isolates of the virus Coat protein (CP) was estimated as 35 kDa by western blotting. Following total RNA extraction from virus infected samples, three discontinuous fragments (corresponding to genomic 3', partial CI and HC-Pro regions) were amplified. The CP region of the virus infecting saffron has the highest nucleotide identity with Ceratobium mosaic virus (CerMV) and Telosma mosaic virus (TelMV) (74%) and the highest deduced amino acid identity with Bean common mosaic necrosis virus (BCMNV) (78. 8%). Despite the high serological relationship with BCMV and a close phylogenetic relationship with BCMV subgroup based on CP region, the identity values are less than described species demarcation criteria for Potyviridae family. Overall, it seems that the saffron infecting Potyvirus is a new species of Potyvirus genus but the definitive statement requires sequencing the entire genome of the virus.

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APA: Copy

Parizad, Shirin, DIZADJI, AKBAR, KOOHI HABIBI, MINA, Mosahebi Mohammadi, Gholam Hossein, KALANTARI, SIAMAK, IZADPANAH, FATEMEH, Garcia Arenal, Fernando, & WINTER, STEPHAN. (2017). Identification and partial characterization of the virus infecting saffron (Crocus sativus) in Iran. IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 47(2 ), 263-275. SID. https://sid.ir/paper/171716/en

Vancouver: Copy

Parizad Shirin, DIZADJI AKBAR, KOOHI HABIBI MINA, Mosahebi Mohammadi Gholam Hossein, KALANTARI SIAMAK, IZADPANAH FATEMEH, Garcia Arenal Fernando, WINTER STEPHAN. Identification and partial characterization of the virus infecting saffron (Crocus sativus) in Iran. IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2017;47(2 ):263-275. Available from: https://sid.ir/paper/171716/en

IEEE: Copy

Shirin Parizad, AKBAR DIZADJI, MINA KOOHI HABIBI, Gholam Hossein Mosahebi Mohammadi, SIAMAK KALANTARI, FATEMEH IZADPANAH, Fernando Garcia Arenal, and STEPHAN WINTER, “Identification and partial characterization of the virus infecting saffron (Crocus sativus) in Iran,” IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 47, no. 2 , pp. 263–275, 2017, [Online]. Available: https://sid.ir/paper/171716/en

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