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Information Journal Paper

Title

GENETIC ENGINEERING FOR INCREASING RESISTANCE OF CHICKPEA (CICER ARIETINUM L.) TO POD BORER (HELICOVERPA ARMIGERA)

Pages

  65-75

Abstract

 Pod borer is one of the main factors for yield decrease of CHICKPEA. Therefore, breeding of CHICKPEA for resistance to this pest is important. Modified CRY1AC gene of Bacillus thuringiensis in pCry1Ac-nptII plasmid containing twin T-DNA for CRY1AC and nptII genes have been used for TRANSFORMATION of CHICKPEA. At last 38 plants had nptII gene that 36 plants of them had CRY1AC gene and two plants had only nptII gene. Western blotting analysis showed that 30 plants had CRY1AC protein expression. TRANSFORMATION percentage was 0.37 in this experiment. Only 7 plants produced T1 seeds. Regarding to western blotting and PCR analysis for T0 and T1, plants were devided to some groups with different expressions. Bioassay with neonate of POD BORER showed high mortality in lines with high CRY1AC protein expression.

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    APA: Copy

    MOSHTAGHI, NASRIN, BAGHERI, A.A.R., HIGINZ, T.J., JALALI JAVARAN, MOKHTAR, & GHARAHYAZI, BEHZAD. (2010). GENETIC ENGINEERING FOR INCREASING RESISTANCE OF CHICKPEA (CICER ARIETINUM L.) TO POD BORER (HELICOVERPA ARMIGERA). IRANIAN JOURNAL OF PULSES RESEARCH, 1(1), 65-75. SID. https://sid.ir/paper/171788/en

    Vancouver: Copy

    MOSHTAGHI NASRIN, BAGHERI A.A.R., HIGINZ T.J., JALALI JAVARAN MOKHTAR, GHARAHYAZI BEHZAD. GENETIC ENGINEERING FOR INCREASING RESISTANCE OF CHICKPEA (CICER ARIETINUM L.) TO POD BORER (HELICOVERPA ARMIGERA). IRANIAN JOURNAL OF PULSES RESEARCH[Internet]. 2010;1(1):65-75. Available from: https://sid.ir/paper/171788/en

    IEEE: Copy

    NASRIN MOSHTAGHI, A.A.R. BAGHERI, T.J. HIGINZ, MOKHTAR JALALI JAVARAN, and BEHZAD GHARAHYAZI, “GENETIC ENGINEERING FOR INCREASING RESISTANCE OF CHICKPEA (CICER ARIETINUM L.) TO POD BORER (HELICOVERPA ARMIGERA),” IRANIAN JOURNAL OF PULSES RESEARCH, vol. 1, no. 1, pp. 65–75, 2010, [Online]. Available: https://sid.ir/paper/171788/en

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