STUDY THE POLYMORPHISM OF EXON 4 OF GH GENE AND EXON 10 OF GHR GENE AND THEIR ASSOCIATION WITH CARCASS TRAITS IN LORI-BAKHTIARI BREED SHEEP USING PCR-SSCP

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MORADI SHAHREBABAK HOSSEIN; KHODABAKHSHZADEH RASOUL; SADEGHI MOSTAFA; TAHERI YEGANEH AMIR

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Journal: Iranian Journal of Animal Science Year:2016 | Volume:47 | Issue:1 Page(s): 51-61

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Title

STUDY THE POLYMORPHISM OF EXON 4 OF GH GENE AND EXON 10 OF GHR GENE AND THEIR ASSOCIATION WITH CARCASS TRAITS IN LORI-BAKHTIARI BREED SHEEP USING PCR-SSCP

Author(s)

MORADI SHAHREBABAK HOSSEIN | KHODABAKHSHZADEH RASOUL | SADEGHI MOSTAFA | TAHERI YEGANEH AMIR | Issue Writer Certificate

Keywords

GROWTH HORMONE GENEQ1

GROWTH HORMONE RECEPTOR GENEQ2

SINGLE NUCLEOTIDE POLYMORPHISMQ2

SEQUENCINGQ2

Abstract

The growth hormone (GH) gene is a candidate for growth trait in farm animals and plays an important role in growth metabolism. Growth hormone can contribute in growth metabolism when it's receptor (GHR) are on cells target. The SINGLE NUCLEOTIDE POLYMORPHISM was occurred in GH and GHR genes and that associated with carcass traits in Lori-Bakhtiari sheep breed with using PCR-SSCP. In this research, blood samples were collected from the left jugular vein from 152 Lori-Bakhtiari sheep breed. Genomic DNA was extracted from blood samples by using the salting-out procedure and PCR were used to amplify the regions are located in exon 4 (214 bp segments) of the ovine GH gene and exon 10 (218 bp segments) of the ovine GHR Gene. The single stranded conformation polymorphism (SSCP) patterns of PCR products were studied using acrylamide gel electrophoresis and silver-nitrate staining method. The result showed polymorphism in GH gene but any difference between banding patterns for GHR gene was not observed. The SEQUENCING results showed the presence of 5 SINGLE NUCLEOTIDE POLYMORPHISM for GH gene in the studied population. No significant associations of the available genotypes in the exon 4 of the ovine GH Gene with carcass traits.

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APA: Copy

MORADI SHAHREBABAK, HOSSEIN, KHODABAKHSHZADEH, RASOUL, SADEGHI, MOSTAFA, & TAHERI YEGANEH, AMIR. (2016). STUDY THE POLYMORPHISM OF EXON 4 OF GH GENE AND EXON 10 OF GHR GENE AND THEIR ASSOCIATION WITH CARCASS TRAITS IN LORI-BAKHTIARI BREED SHEEP USING PCR-SSCP. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 47(1), 51-61. SID. https://sid.ir/paper/193507/en

Vancouver: Copy

MORADI SHAHREBABAK HOSSEIN, KHODABAKHSHZADEH RASOUL, SADEGHI MOSTAFA, TAHERI YEGANEH AMIR. STUDY THE POLYMORPHISM OF EXON 4 OF GH GENE AND EXON 10 OF GHR GENE AND THEIR ASSOCIATION WITH CARCASS TRAITS IN LORI-BAKHTIARI BREED SHEEP USING PCR-SSCP. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2016;47(1):51-61. Available from: https://sid.ir/paper/193507/en

IEEE: Copy

HOSSEIN MORADI SHAHREBABAK, RASOUL KHODABAKHSHZADEH, MOSTAFA SADEGHI, and AMIR TAHERI YEGANEH, “STUDY THE POLYMORPHISM OF EXON 4 OF GH GENE AND EXON 10 OF GHR GENE AND THEIR ASSOCIATION WITH CARCASS TRAITS IN LORI-BAKHTIARI BREED SHEEP USING PCR-SSCP,” IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 47, no. 1, pp. 51–61, 2016, [Online]. Available: https://sid.ir/paper/193507/en

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