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Information Journal Paper

Title

The potential of catalase as an enzymatic antioxidant to improve freezability of Turkmen stallions sperm

Pages

  215-222

Abstract

 Four mature stallions were used to study the effect of enzymatic antioxidant Catalase on freezability of Turkmen stallion's sperm. Collected semen from stallions were processed and pooled before being divided treatments (200×106 sperm/ml) and diluted with extenders supplemented with different levels of Catalase [0 (control), 100 U/ml (CAT100), 150U/ml (CAT150) and 200U/ml (CAT200)]. Extended and supplemented semen's were frozen according to a standard protocol. After thawing, motility, viability, membrane integrity, total abnormality and lipid peroxidation were assessed. Results showed that addition of 200U/ml Catalase could improve progressive and total motility, viability, membrane integrity and decreased lipid peroxidation compared to control and other levels of Catalase (p<0. 05). In addition, 150 U/ml Catalase improved total motility, viability and decreased MDA concentration (p<0. 05). However, the addition of 100 U/ml Catalase had no significant effect on sperm quality (p>0. 05). The results showed that different levels of Catalase could not decreased total abnormality (p>0. 05). We concluded that addition of 200U/ml Catalase could improve freezability of Turkmen stallion’ s sperm.

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  • Cite

    APA: Copy

    SEIFI JAMADI, AFSHIN, ZARE SHAHNEH, AHMAD, KOHRAM, HAMID, AKBARI, ABOLFAZL, ZAMEN, MOHSEN, & Vakhideh, Abolfazl. (2016). The potential of catalase as an enzymatic antioxidant to improve freezability of Turkmen stallions sperm. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 47(2 ), 215-222. SID. https://sid.ir/paper/193556/en

    Vancouver: Copy

    SEIFI JAMADI AFSHIN, ZARE SHAHNEH AHMAD, KOHRAM HAMID, AKBARI ABOLFAZL, ZAMEN MOHSEN, Vakhideh Abolfazl. The potential of catalase as an enzymatic antioxidant to improve freezability of Turkmen stallions sperm. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2016;47(2 ):215-222. Available from: https://sid.ir/paper/193556/en

    IEEE: Copy

    AFSHIN SEIFI JAMADI, AHMAD ZARE SHAHNEH, HAMID KOHRAM, ABOLFAZL AKBARI, MOHSEN ZAMEN, and Abolfazl Vakhideh, “The potential of catalase as an enzymatic antioxidant to improve freezability of Turkmen stallions sperm,” IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 47, no. 2 , pp. 215–222, 2016, [Online]. Available: https://sid.ir/paper/193556/en

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