Effects of different centrifugation protocols on pre and post-cooling sperm quality in Caspian horses

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Nouri Houshang; TOWHIDI ARMIN; Bahreini Majid; Nadri Touba

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Journal: Iranian Journal of Animal Science Year:2017 | Volume:47 | Issue:4 Page(s): 481-487

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Information Journal Paper

Title

Effects of different centrifugation protocols on pre and post-cooling sperm quality in Caspian horses

Author(s)

Nouri Houshang | TOWHIDI ARMIN | Bahreini Majid | Nadri Touba | Issue Writer Certificate

Keywords

HealthyQ2

matilityQ2

quality evaluationQ2

spermQ1

viabilityQ1

Abstract

This study aimed to determine the effects of different centrifuging protocols (different rotation and time) on Caspian horse sperm characteristics. In this study, semen samples from four Healthy adult Caspian pony stalian were collected using an artificial vagina and then diluted in milk phosphocaseinat based extender. Then semen samples were divided into seven equal parts, which part assigned to treatments including centrifugation treatments (500×g, 1000×g and 2000×g each for 4 and 7 minutes) and without centrifuge (control). Cooling of treatments were done at 0, 24 and 48 hours after centrifugation. sperm motion characteristics, viability and percentages of Healthy sperm were assessed. The results showed that motility, viability and percentages of Healthy sperm reduced in 2000×g treatments (p<0. 05). Stored sperm in 500×g and 1000×g treatments had better quality compared to control group (p<0. 05). In addition, compared to 2000×g treatment, in 500×g and 1000×g treatments a less recovery rate was observed (p<0. 05). In conclusion, Caspian horse's semen in treatment with centrifuging for 7 minutes at 1000 g, showed better result.

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APA: Copy

Nouri, Houshang, TOWHIDI, ARMIN, Bahreini, Majid, & Nadri, Touba. (2017). Effects of different centrifugation protocols on pre and post-cooling sperm quality in Caspian horses. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 47(4 ), 481-487. SID. https://sid.ir/paper/193647/en

Vancouver: Copy

Nouri Houshang, TOWHIDI ARMIN, Bahreini Majid, Nadri Touba. Effects of different centrifugation protocols on pre and post-cooling sperm quality in Caspian horses. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2017;47(4 ):481-487. Available from: https://sid.ir/paper/193647/en

IEEE: Copy

Houshang Nouri, ARMIN TOWHIDI, Majid Bahreini, and Touba Nadri, “Effects of different centrifugation protocols on pre and post-cooling sperm quality in Caspian horses,” IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 47, no. 4 , pp. 481–487, 2017, [Online]. Available: https://sid.ir/paper/193647/en

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