STUDY OF GENETIC VARIATION IN IRANIAN KURDISH HORSE USING MICROSATELLITE MARKER

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ALA AMJADI MOTAHAREH; MEHRABANI YEGANEH HASSAN; SADEGHI MOSTAFA

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Journal: Iranian Journal of Animal Science Year:2017 | Volume:48 | Issue:3 Page(s): 335-342

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Title

STUDY OF GENETIC VARIATION IN IRANIAN KURDISH HORSE USING MICROSATELLITE MARKER

Author(s)

ALA AMJADI MOTAHAREH | MEHRABANI YEGANEH HASSAN | SADEGHI MOSTAFA | Issue Writer Certificate

Keywords

KURDISH HORSEQ1

MICROSATELLITE MARKERSQ1

POLYMORPHISMSQ2

Abstract

In this research genetic variation using 6 microsatellite loci (HMS07, HMS03, HMS02, HMS06, ASB02, ASB23) was studied in Iranian KURDISH HORSE population. Whole blood samples were randomly collected from 52 horses in Kurdistan, Kermanshah, Elam, Western Azerbaijan, Esfahan, Kerman and Hamadan regions. DNA was extracted from blood samples. Polymerase chain reaction (PCR) was used for amplification of 6 microsatellite loci using pairs of standard specific primers. Then, the PCR products assessed with 8% silver-staining acrylamide gel and electrophoresed. All microsatellites loci were polymorphic. The number of alleles observed and expected hetrozygosities were calculated with Shannon Index. The largest and smallest number of alleles were observed in HMS07 locus (10 alleles) and HMS02 (4 alleles) respectively. Our results confirmed the high efficiency of MICROSATELLITE MARKERS for assessing population structure in Iranian native horses.

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APA: Copy

ALA AMJADI, MOTAHAREH, MEHRABANI YEGANEH, HASSAN, & SADEGHI, MOSTAFA. (2017). STUDY OF GENETIC VARIATION IN IRANIAN KURDISH HORSE USING MICROSATELLITE MARKER. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 48(3 ), 335-342. SID. https://sid.ir/paper/193710/en

Vancouver: Copy

ALA AMJADI MOTAHAREH, MEHRABANI YEGANEH HASSAN, SADEGHI MOSTAFA. STUDY OF GENETIC VARIATION IN IRANIAN KURDISH HORSE USING MICROSATELLITE MARKER. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2017;48(3 ):335-342. Available from: https://sid.ir/paper/193710/en

IEEE: Copy

MOTAHAREH ALA AMJADI, HASSAN MEHRABANI YEGANEH, and MOSTAFA SADEGHI, “STUDY OF GENETIC VARIATION IN IRANIAN KURDISH HORSE USING MICROSATELLITE MARKER,” IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 48, no. 3 , pp. 335–342, 2017, [Online]. Available: https://sid.ir/paper/193710/en

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