ASSESSMENT OF THE APPROPRIATE CONDITIONS FOR MAINTAIN OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) BLOOD SAMPLES WITH THE GOAL OF BIOMONITORING VIA COMET ASSAY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOODI SAEED; FARAHMAND HAMID; MIRVAGHEFI ALIREZA; KOSARI ALI ASGHAR; KHALILI BITA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Natural Environment Year:2013 | Volume:66 | Issue:2 Page(s): 203-212

Download Full-Text

Persian Verion

View:

592

Download:

Cites:

Information Journal Paper

Title

ASSESSMENT OF THE APPROPRIATE CONDITIONS FOR MAINTAIN OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) BLOOD SAMPLES WITH THE GOAL OF BIOMONITORING VIA COMET ASSAY

Author(s)

Keywords

BLOOD CELLQ2

COMET ASSAYQ2

DNA DAMAGEQ2

GENOTOXICITY BIOMARKERQ2

PRESERVATION MEDIAQ2

Abstract

Biomonitoring is the main way to assess the pollution status in aquatic ecosystems where Biomarkers response is used at different levels of trace contaminants on the aquatic organisms. Recently, many investigations have been focused to the genotoxic biomarker in aquatic ecosystems owing to mutagen effects of the contaminant on such environment. However, one of the fundamental problems in this area is the lack of proper maintenance protocol for transferring of biological samples with minimum damage to the laboratory. For this purpose, it is required to use appropriate conditions evaluation and standardization to avoid any possible test error analysis of the habitat situation in this matter. In this study, the COMET ASSAY as a new method in Biomarker studies at the molecular level that is based on measuring damages reflected as strand breakage, was established to investigate the effects of three storage solutions (FBS, L-15 and PBS) on DNA integrity in red BLOOD CELL of rainbow trout (Oncorhynchus mykiss) at different times after blood sampling (6, 12, 24 and 48 h). Cell viability as well as was determined by trypan blue dye exclusion in time intervals. The Results of COMET ASSAY in blood samples which has been maintained in L-15 and FBS solutions, did not exhibit significant differences in DNA DAMAGE between different times, while samples preserved 24 h in PBS indicated the significant increase in the levels of DNA DAMAGE compared to time 0 as control (P<0.05). According to the results in this study, it is appeared that media osmolarity is the most important factor in maintaining viability of red BLOOD CELLs during storage in different solutions. Overall, this result indicates that the blood samples from freshwater fish can be preserved up to 48 h in FBS or L-15 mediums at 4oC in the absence of light with no significant DNA DAMAGE.

Cites

No record.

References

No record.

Cite

APA: Copy

MOODI, SAEED, FARAHMAND, HAMID, MIRVAGHEFI, ALIREZA, KOSARI, ALI ASGHAR, & KHALILI, BITA. (2013). ASSESSMENT OF THE APPROPRIATE CONDITIONS FOR MAINTAIN OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) BLOOD SAMPLES WITH THE GOAL OF BIOMONITORING VIA COMET ASSAY. JOURNAL OF NATURAL ENVIRONMENT (IRANIAN JOURNAL OF NATURAL RESOURCES), 66(2), 203-212. SID. https://sid.ir/paper/194817/en

Vancouver: Copy

MOODI SAEED, FARAHMAND HAMID, MIRVAGHEFI ALIREZA, KOSARI ALI ASGHAR, KHALILI BITA. ASSESSMENT OF THE APPROPRIATE CONDITIONS FOR MAINTAIN OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) BLOOD SAMPLES WITH THE GOAL OF BIOMONITORING VIA COMET ASSAY. JOURNAL OF NATURAL ENVIRONMENT (IRANIAN JOURNAL OF NATURAL RESOURCES)[Internet]. 2013;66(2):203-212. Available from: https://sid.ir/paper/194817/en

IEEE: Copy

SAEED MOODI, HAMID FARAHMAND, ALIREZA MIRVAGHEFI, ALI ASGHAR KOSARI, and BITA KHALILI, “ASSESSMENT OF THE APPROPRIATE CONDITIONS FOR MAINTAIN OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) BLOOD SAMPLES WITH THE GOAL OF BIOMONITORING VIA COMET ASSAY,” JOURNAL OF NATURAL ENVIRONMENT (IRANIAN JOURNAL OF NATURAL RESOURCES), vol. 66, no. 2, pp. 203–212, 2013, [Online]. Available: https://sid.ir/paper/194817/en

Related Journal Papers