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Title

THE SEQUENCES DETERMINATION OF 16SRRNA GENE FOR IDENTIFICATION AND PHYLOGENIC STUDY ON RHIZOBIUM BACTERIA

Pages

  12-14

Abstract

 The rRNA genes are essential for the survival of all organisms and also the sequences of these genes are highly conserved. For this reason, the determination of 168 RRNA GENE sequences is used as a standard method for the identification of species, genera and families of bacteria. Recently, the small parts of the rRNA gene sequences are used for bacterial assessing at the level of genus and above. The data obtained from the sequences of the small parts of 168 rRNA suggest that RHIZOBIUM genus can be split into four genera. In this investigation, two rhizobial isolates were isolated from the hairy vetch plant nodules (Viciae hirsuta) and they had different PLASMID PROFILE types. By using two specific primers, the first part of the 168 RRNA GENE (about 230 base pairs) were amplified by using PCR technique. The 168 RRNA GENE sequences of two isolates were compared with those in the gene bank database. The results showed that the sequences of two isolate had 100% homology with those in Rhizobuim leguminosarum' bv. Viciae. Therefore, The sequences determination of 168 RRNA GENE is a useful and reliable method for rhizobia identification.

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    APA: Copy

    LAKZIAN, AMIR. (2001). THE SEQUENCES DETERMINATION OF 16SRRNA GENE FOR IDENTIFICATION AND PHYLOGENIC STUDY ON RHIZOBIUM BACTERIA. PAJOUHESH-VA-SAZANDEGI, 13(4 (49)), 12-14. SID. https://sid.ir/paper/19633/en

    Vancouver: Copy

    LAKZIAN AMIR. THE SEQUENCES DETERMINATION OF 16SRRNA GENE FOR IDENTIFICATION AND PHYLOGENIC STUDY ON RHIZOBIUM BACTERIA. PAJOUHESH-VA-SAZANDEGI[Internet]. 2001;13(4 (49)):12-14. Available from: https://sid.ir/paper/19633/en

    IEEE: Copy

    AMIR LAKZIAN, “THE SEQUENCES DETERMINATION OF 16SRRNA GENE FOR IDENTIFICATION AND PHYLOGENIC STUDY ON RHIZOBIUM BACTERIA,” PAJOUHESH-VA-SAZANDEGI, vol. 13, no. 4 (49), pp. 12–14, 2001, [Online]. Available: https://sid.ir/paper/19633/en

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