MOLECULAR ISOLATION OF SALMONELLA FROM FAECES OF THE STREET CAT BY PCR METHOD

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SADEGHI VAFA F.; MASHHADI RAFIEI S.; JAMSHIDIAN M.

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Paper Information

Journal: JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN Year:2017 | Volume:14 (NEW) | Issue:1 (56) Page(s): 2113-2119

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Title

MOLECULAR ISOLATION OF SALMONELLA FROM FAECES OF THE STREET CAT BY PCR METHOD

Author(s)

SADEGHI VAFA F. | MASHHADI RAFIEI S. | JAMSHIDIAN M. | Issue Writer Certificate

Keywords

SALMONELLA ENTERITIDISQ2

SEROTYPINGQ2

CATQ2

TEHRANQ3

Abstract

This study was performed for detecting enteric Salmonella in stray CATs in TEHRAN. Stool samples obtained from 100 stray CATs were referred to small animal clinics by SPCA members from different part of TEHRAN. Routine culture, SEROTYPING test and molecular methods (PCR and MPCR) were done for evaluating of genius and serovars of bacteria. Samples were placed in 37oC for 24 hours in selective media such as Rambach medium. After the colonies appeared, they cultured in differential media such as TSI. Results recorded after 24 hours later. Only one sample showed Salmonella Spp. in culture method from a 2 month old kitten with hemorrhagic enteritis and severe dehydration. This sample and other suspected cases were tested by PCR and M-PCR subsequently. We found another case of SALMONELLA ENTERITIDIS from a female one-yearold CAT without any clinical signs. SEROTYPING tests showed both cases belong to groupD1. Although SALMONELLA ENTERITIDIS is an important factor in human gastroenteritis and recorded from poultry farms and products, it is supposed to detecting this kind of Salmonella are created after exposing to related resources.

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APA: Copy

SADEGHI VAFA, F., MASHHADI RAFIEI, S., & JAMSHIDIAN, M.. (2017). MOLECULAR ISOLATION OF SALMONELLA FROM FAECES OF THE STREET CAT BY PCR METHOD. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, 14 (NEW)(1 (56)), 2113-2119. SID. https://sid.ir/paper/197390/en

Vancouver: Copy

SADEGHI VAFA F., MASHHADI RAFIEI S., JAMSHIDIAN M.. MOLECULAR ISOLATION OF SALMONELLA FROM FAECES OF THE STREET CAT BY PCR METHOD. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN[Internet]. 2017;14 (NEW)(1 (56)):2113-2119. Available from: https://sid.ir/paper/197390/en

IEEE: Copy

F. SADEGHI VAFA, S. MASHHADI RAFIEI, and M. JAMSHIDIAN, “MOLECULAR ISOLATION OF SALMONELLA FROM FAECES OF THE STREET CAT BY PCR METHOD,” JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, vol. 14 (NEW), no. 1 (56), pp. 2113–2119, 2017, [Online]. Available: https://sid.ir/paper/197390/en

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