RAPID DIFFERENTIATION OF AVIAN INFECTIOUS BRONCHITIS VIRUS STRAINS BY HRM ANALYSIS

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MASOUDIAN A.; SHEIKHI N.; BOZORGHMEHRI FARD M.H.

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ISSN: 2588-4824

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Paper Information

Journal: JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN Year:2013 | Volume:10 (NEW) | Issue:1 (40) Page(s): 899-905

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Title

RAPID DIFFERENTIATION OF AVIAN INFECTIOUS BRONCHITIS VIRUS STRAINS BY HRM ANALYSIS

Author(s)

MASOUDIAN A. | SHEIKHI N. | BOZORGHMEHRI FARD M.H. | Issue Writer Certificate

Keywords

INFECTIOUS BRONCHITIS VIRUSQ2

RAPID DIFFERENTIATIONQ2

HRM ANALYSISQ2

Abstract

Infectious Bronchitis is an acute contagious Coronovirus infection in chicken flocks that causes upper respiratory, renal and reproductive diseases in infected premises. Detection and differentiation of causative agent is essential to plan prevention and control strategies such as vaccination. Because most of detection techniques are time consuming, a real-time polymerase chain reaction (RT-PCR) combined with high-resolution melt (HRM) curve analysis was developed based on the 3’UTR sequence of vaccinal strains as references of IBV to establish a rapid detection and differentiation method for IBV strains. Results showed that the vaccinal reference strains were divided in four groups according to melt curve models for 400 to 430 bp PCR products and calculating Genotype Confidence Percentage (GCP). This Study resulted in an introduction of a geometric and arithmetic model for rapid and reliable classification of IBV strains base on 3’UTR HRM ANALYSIS.

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