COMPARISON OF THE VALIDITY OF CIE AND PCR DIAGNOSTIC METHODS IN DETERMINING HYDATIDOSIS IN SHEEP

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YAKHCHALI M.; MORSHEDI A.; SHIRASHIAN M.

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Journal: Veterinary Research & Biological Products Year:2012 | Volume:25 | Issue:1 (94) Page(s): 25-30

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Information Journal Paper

Title

COMPARISON OF THE VALIDITY OF CIE AND PCR DIAGNOSTIC METHODS IN DETERMINING HYDATIDOSIS IN SHEEP

Author(s)

YAKHCHALI M. | MORSHEDI A. | SHIRASHIAN M. | Issue Writer Certificate

Keywords

HYDATID CYSTQ2

SHEEPQ2

CIEQ2

PCRQ2

Abstract

Sheep is one of the important hosts for hydatidosis in Iran as a hyperendemic region worldwide. In the present study, the efficacy of counter immuno-electerophoresis (CIE) and PCR tests to detect SHEEP HYDATID CYST infection was carried out. For this, soluble antigen was provided using HYDATID CYST fluids (HCFs). Blood sera were taken from a total number of 100 slaughtered SHEEP. CIE analysis was undertaken using negative and positive controls. DNA was extracted from protoscolices and a 1213bp fragment length of co1 gene amplified. Necropsy findings indicated that lung and liver of slaughtered SHEEP (33%) was infected with HYDATID CYST. While it was 29% in CIE and 28% in PCR test. According to the necropsy findings as a gold standard test, sensitivity and specificity of CIE was 79.31% and 92.2%, respectively. Sensitivity and specifity of PCR test were determined 85.19% and 94.8%, as well. Data analysis showed that there is no significant difference between these two techniques (P<0.05). Based on these results, CIE technique can be considered as a useful method to screen SHEEP HYDATID CYST infection.

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APA: Copy

YAKHCHALI, M., MORSHEDI, A., & SHIRASHIAN, M.. (2012). COMPARISON OF THE VALIDITY OF CIE AND PCR DIAGNOSTIC METHODS IN DETERMINING HYDATIDOSIS IN SHEEP. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), 25(1 (94)), 25-30. SID. https://sid.ir/paper/201047/en

Vancouver: Copy

YAKHCHALI M., MORSHEDI A., SHIRASHIAN M.. COMPARISON OF THE VALIDITY OF CIE AND PCR DIAGNOSTIC METHODS IN DETERMINING HYDATIDOSIS IN SHEEP. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI)[Internet]. 2012;25(1 (94)):25-30. Available from: https://sid.ir/paper/201047/en

IEEE: Copy

M. YAKHCHALI, A. MORSHEDI, and M. SHIRASHIAN, “COMPARISON OF THE VALIDITY OF CIE AND PCR DIAGNOSTIC METHODS IN DETERMINING HYDATIDOSIS IN SHEEP,” VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), vol. 25, no. 1 (94), pp. 25–30, 2012, [Online]. Available: https://sid.ir/paper/201047/en

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