THE SURVEY EFFECT OF BAP AND TDZ ON DIRECT SHOOT REGENERATION FROM NODAL EXPLANT OF AGASTACHE FOENICULUM

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HOSSEINI BAHMAN; MOHARAMI LEILA

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Paper Information

Journal: JOURNAL OF CROPS IMPROVEMENT (JOURNAL OF AGRICULTURE) Year:2014 | Volume:16 | Issue:2 Page(s): 459-473

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Title

THE SURVEY EFFECT OF BAP AND TDZ ON DIRECT SHOOT REGENERATION FROM NODAL EXPLANT OF AGASTACHE FOENICULUM

Author(s)

HOSSEINI BAHMAN | MOHARAMI LEILA | Issue Writer Certificate

Keywords

AGASTACHE FOENICULUMQ2

INDOLE-3-BUTYRIC ACIDQ2

IN VITROQ3

METHYL CHAVICOLQ2

SHOOT INDUCTIONQ2

Abstract

The first successful and an efficient regeneration system on IN VITRO direct plant regeneration of AGASTACHE FOENICULUM was reported. The experiment were done in factorial design with three replications and explants were cultured in MS media fortified with different concentrations of BAP and TDZ (0, 2.2, 4.4, 8.8 mM) in combination with 3 levels of IAA (0, 1.1 and 2.2 mM). ANOVA analysis showed that direct SHOOT INDUCTION and regeneration was significantly influenced by hormones combinations. In BAP and IAA treatments, highest frequency of SHOOT INDUCTION (32.72) and maximum regenerated shoots (7.9 shoots per explants) was observed in MS media containing BAP (8.8 mM) alone. Although in TDZ and IAA treatments, highest average number of induced shoots (24.6) and (7.13 shoots per explants) were obtained in MS media fortified with 2.2 mM of TDZ. In second experiments, effect of basal media and different concentrations of IAA and IBA on root induction was analyzed. Profuse rooting was obtained in 74 percent of plantlets on 1.2 MS media containing (1.1mM) IBA and highest mean rooting (5.06 roots per seedlings) were obtained in same media. The well rooted plantlets were acclimatized successfully in greenhouse on perlite media and transferred to soil with 100 percent survival.

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APA: Copy

HOSSEINI, BAHMAN, & MOHARAMI, LEILA. (2014). THE SURVEY EFFECT OF BAP AND TDZ ON DIRECT SHOOT REGENERATION FROM NODAL EXPLANT OF AGASTACHE FOENICULUM. JOURNAL OF CROPS IMPROVEMENT (JOURNAL OF AGRICULTURE), 16(2), 459-473. SID. https://sid.ir/paper/209339/en

Vancouver: Copy

HOSSEINI BAHMAN, MOHARAMI LEILA. THE SURVEY EFFECT OF BAP AND TDZ ON DIRECT SHOOT REGENERATION FROM NODAL EXPLANT OF AGASTACHE FOENICULUM. JOURNAL OF CROPS IMPROVEMENT (JOURNAL OF AGRICULTURE)[Internet]. 2014;16(2):459-473. Available from: https://sid.ir/paper/209339/en

IEEE: Copy

BAHMAN HOSSEINI, and LEILA MOHARAMI, “THE SURVEY EFFECT OF BAP AND TDZ ON DIRECT SHOOT REGENERATION FROM NODAL EXPLANT OF AGASTACHE FOENICULUM,” JOURNAL OF CROPS IMPROVEMENT (JOURNAL OF AGRICULTURE), vol. 16, no. 2, pp. 459–473, 2014, [Online]. Available: https://sid.ir/paper/209339/en

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