GENETIC STRUCTURE OF A PERSIAN PARTRIDGE POPULATION IN ANIMAL SCIENCE RESEARCH INSTITUTE OF IRAN USING MICROSATELLITE MARKERS

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BOROUFEH K.; AMIRINIA C.; EMRANI H.; NOSHARI A.R.

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Journal: Animal Science Journal Year:2011 | Volume:24 | Issue:3 (92) Page(s): 31-40

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Title

GENETIC STRUCTURE OF A PERSIAN PARTRIDGE POPULATION IN ANIMAL SCIENCE RESEARCH INSTITUTE OF IRAN USING MICROSATELLITE MARKERS

Author(s)

BOROUFEH K. | AMIRINIA C. | EMRANI H. | NOSHARI A.R. | Issue Writer Certificate

Keywords

ALECTORIS CHUKARQ3

PERSIAN PARTRIDGEQ3

MICROSATELLITE MARKERSQ2

GENETIC VARIATIONQ2

Abstract

The aim of this study was take advantages of usefulness potential MICROSATELLITE MARKERS in detect PERSIAN PARTRIDGE Genetic Structure and population variability for the first time. The chukar partridge population was collected from Semnan & Fars province that keeping in Animal Science Research Institute of Iran (ASRI).The number of 120 blood samples were collected and DNA was isolated using Optimized and Modified Salting-out method. eight MICROSATELLITE MARKERS using in this study was Aru1F114, Aru1E97, Aru1E102, Aru1E66, Aru1B3, Aru1G4, Aru1M127 & Aru1J76.The elements and thermal cycle for each locus was optimized before done PCR reaction. After PCR amplification, microsatellite alleles were identified on 8% denaturing polyacrylamide gels, followed by AgNO3 staining so partridges had been genotyped. Data analyses consist of several parameters of population genetics were done using GENEPOP 3.1.All of locus tested for Hardy-Weinberg equilibrium by chi-square (X2) and likelihood ratio (G2). All loci except Aru1E102 were deviated from Hardy-Weinberg equilibrium in population (P<0.05). The entire loci were polymorphic. The most number of observed alleles were in Aru1E97 with 14 alleles and the lowest number of alleles were in Aru1F114, Aru1M127 & Aru1J76 with 4 alleles. The average observed number of alleles, Shannon’s Information index & Polymorphic Information Content (PIC) were 6.3750, 1.4568 and 0.6567 respectively. The average observed heterozygosity was 0.4522 and average expected heterozygosity was obtained 0.7017.Results of GENETIC VARIATION in PERSIAN PARTRIDGE population as comparison with other exotic similar studies, detect suitable variation value in Persian native partridge population.

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APA: Copy

BOROUFEH, K., AMIRINIA, C., EMRANI, H., & NOSHARI, A.R.. (2011). GENETIC STRUCTURE OF A PERSIAN PARTRIDGE POPULATION IN ANIMAL SCIENCE RESEARCH INSTITUTE OF IRAN USING MICROSATELLITE MARKERS. ANIMAL SCIENCES JOURNAL (PAJOUHESH & SAZANDEGI), 24(3 (92)), 31-40. SID. https://sid.ir/paper/215016/en

Vancouver: Copy

BOROUFEH K., AMIRINIA C., EMRANI H., NOSHARI A.R.. GENETIC STRUCTURE OF A PERSIAN PARTRIDGE POPULATION IN ANIMAL SCIENCE RESEARCH INSTITUTE OF IRAN USING MICROSATELLITE MARKERS. ANIMAL SCIENCES JOURNAL (PAJOUHESH & SAZANDEGI)[Internet]. 2011;24(3 (92)):31-40. Available from: https://sid.ir/paper/215016/en

IEEE: Copy

K. BOROUFEH, C. AMIRINIA, H. EMRANI, and A.R. NOSHARI, “GENETIC STRUCTURE OF A PERSIAN PARTRIDGE POPULATION IN ANIMAL SCIENCE RESEARCH INSTITUTE OF IRAN USING MICROSATELLITE MARKERS,” ANIMAL SCIENCES JOURNAL (PAJOUHESH & SAZANDEGI), vol. 24, no. 3 (92), pp. 31–40, 2011, [Online]. Available: https://sid.ir/paper/215016/en

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