THE POLYMORPHISM OF EXON 2 OF GOLA-DRB3 GENE IN NADUSHAN GOAT USING PCR-RFLP

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ABBASZADEH E.; MOHAMMADABADI M.R.; ESMAILIZADEH A.; ALINAGHIZADEH R.

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Journal: Iranian Journal of Animal Science Research Year:2011 | Volume:3 | Issue:3 Page(s): 274-279

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Title

THE POLYMORPHISM OF EXON 2 OF GOLA-DRB3 GENE IN NADUSHAN GOAT USING PCR-RFLP

Author(s)

ABBASZADEH E. | MOHAMMADABADI M.R. | ESMAILIZADEH A. | ALINAGHIZADEH R. | Issue Writer Certificate

Keywords

GOLA-DRB3 GENEQ3

NADUSHAN GOATQ2

PCR-RFLPQ3

Abstract

This study was conducted to determine polymorphism of GOLA-DRB3 in NADUSHAN GOAT using PCR-RFLP.Blood samples were randomly taken from 100 goats. DNA was extracted from the blood samples using DIA tom DNA prep Kit. Spectrophotometer and 1% agarose gel were used for determining its quantities and qualities.Exon 2 of DRB 3.2 gene encompassing 285 bp was amplified with heminested-PCR method in two rounds and PCR products were digested withTaq I. Digested PCR products were electrophoresed in 10% Acryl Amide gel or 2% Agarose gel and gels stained with ethidium bromide. Digested PCR products withTaq I included 2 fragments at 122bp and 163bp (T restriction pattern) or undigested fragment at 285bp (t restriction pattern). Population was in Hardy-Weinberg equilibrium (p>0.05). Shanon index, Nei index, observed and expected heterozygosity were 0.68, 0.49, 0.55 and 0.49, respectively. With regard to results of this study, GOLA-DRB3 marker has good efficiency for genetic diversity determining. Using results of this study and former studies and records and quantitative information of locus in this population, we can perform determining and identifying QTL.

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APA: Copy

ABBASZADEH, E., MOHAMMADABADI, M.R., ESMAILIZADEH, A., & ALINAGHIZADEH, R.. (2011). THE POLYMORPHISM OF EXON 2 OF GOLA-DRB3 GENE IN NADUSHAN GOAT USING PCR-RFLP. IRANIAN JOURNAL OF ANIMAL SCIENCE RESEARCH, 3(3), 274-279. SID. https://sid.ir/paper/218393/en

Vancouver: Copy

ABBASZADEH E., MOHAMMADABADI M.R., ESMAILIZADEH A., ALINAGHIZADEH R.. THE POLYMORPHISM OF EXON 2 OF GOLA-DRB3 GENE IN NADUSHAN GOAT USING PCR-RFLP. IRANIAN JOURNAL OF ANIMAL SCIENCE RESEARCH[Internet]. 2011;3(3):274-279. Available from: https://sid.ir/paper/218393/en

IEEE: Copy

E. ABBASZADEH, M.R. MOHAMMADABADI, A. ESMAILIZADEH, and R. ALINAGHIZADEH, “THE POLYMORPHISM OF EXON 2 OF GOLA-DRB3 GENE IN NADUSHAN GOAT USING PCR-RFLP,” IRANIAN JOURNAL OF ANIMAL SCIENCE RESEARCH, vol. 3, no. 3, pp. 274–279, 2011, [Online]. Available: https://sid.ir/paper/218393/en

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