DETECTION OF TYPE A OF BEET NECROTIC YELLOWVEIN VIRUS (BNYVV) USING SPECIFIC PRIMERS IN RTPCR METHOD IN RAZAVI KHORASAN PROVINCE

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GHAROONI KARDANI S.; JAFARPOUR B.; FALAHATI RASTEGAR M.; TABASINEZHAD F.

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Paper Information

Journal: Agricultural Sciences and Technology Year:2007 | Volume:21 | Issue:1 (SPECIAL ISSUE IN PLANT PROTECTION) Page(s): 39-47

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Title

DETECTION OF TYPE A OF BEET NECROTIC YELLOWVEIN VIRUS (BNYVV) USING SPECIFIC PRIMERS IN RTPCR METHOD IN RAZAVI KHORASAN PROVINCE

Author(s)

GHAROONI KARDANI S. | JAFARPOUR B. | FALAHATI RASTEGAR M. | TABASINEZHAD F. | Issue Writer Certificate

Keywords

BNYVVQ4

DAS-ELISAQ3

POLYMERASE CHAIN REACTIONQ3

TYPE A.Q3

Abstract

Beet necrotic yellow vein virus (BNYVV) is the type member of the genus Benyvirus which causes the disease known as Rhizomania.From an economic point of view, it is the most important sugar beet virus transmitted by a soil borne fungus, Polymyxa betae Keskin and the vector survives in soil for many years. BNYVV is characterized by rod-shaped particles and has four types known as: A, B, P and J. Types A and B consist only four plus-sense strands of RNA and types P and J contain RNA5 as well. Samples with Rhizomania symptoms were collected from different fields in the main sugar beet growing areas of Razavi Khorasan province in the summer of 2005. Detection of infected samples were Carried out by DAS-ELISA. To do RT-PCR test, total RNA was extracted from roots of the infected samples by using PEG6000 precipitation method and the cDNA was synthesised using random hexamer primers. PCR was performed with specific forward and reverse primers from TGB region on RNA2.After electrophoresis on 1.5% agarose and 5% polyacrylamide gel, the band of 324 bp was detected. This amplified region was specific for TYPE A.

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References

وجود گسترده بیماری ریشه ریشی چغندرقند (شبه Rhizomania) در فارس

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APA: Copy

GHAROONI KARDANI, S., JAFARPOUR, B., FALAHATI RASTEGAR, M., & TABASINEZHAD, F.. (2007). DETECTION OF TYPE A OF BEET NECROTIC YELLOWVEIN VIRUS (BNYVV) USING SPECIFIC PRIMERS IN RTPCR METHOD IN RAZAVI KHORASAN PROVINCE. AGRICULTURAL SCIENCES AND TECHNOLOGY, 21(1 (SPECIAL ISSUE IN PLANT PROTECTION)), 39-47. SID. https://sid.ir/paper/22327/en

Vancouver: Copy

GHAROONI KARDANI S., JAFARPOUR B., FALAHATI RASTEGAR M., TABASINEZHAD F.. DETECTION OF TYPE A OF BEET NECROTIC YELLOWVEIN VIRUS (BNYVV) USING SPECIFIC PRIMERS IN RTPCR METHOD IN RAZAVI KHORASAN PROVINCE. AGRICULTURAL SCIENCES AND TECHNOLOGY[Internet]. 2007;21(1 (SPECIAL ISSUE IN PLANT PROTECTION)):39-47. Available from: https://sid.ir/paper/22327/en

IEEE: Copy

S. GHAROONI KARDANI, B. JAFARPOUR, M. FALAHATI RASTEGAR, and F. TABASINEZHAD, “DETECTION OF TYPE A OF BEET NECROTIC YELLOWVEIN VIRUS (BNYVV) USING SPECIFIC PRIMERS IN RTPCR METHOD IN RAZAVI KHORASAN PROVINCE,” AGRICULTURAL SCIENCES AND TECHNOLOGY, vol. 21, no. 1 (SPECIAL ISSUE IN PLANT PROTECTION), pp. 39–47, 2007, [Online]. Available: https://sid.ir/paper/22327/en

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