SSR MARKERS FOR SCREENING AND ESTIMATING GENETIC DISTANCE OF HOMOKARYOTIC SINGLE SPORES IN THE WHITE BUTTON MUSHROOM (AGARICUS BISPORUS)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BEYRATI M.; MALEKZADEH SHAFAROUDI S.; MALEKZADEH K.; MIRSHAMSI KAKHKI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2015 | Volume:6 | Issue:4 Page(s): 23-36

Download Full-Text

Persian Verion

View:

914

Download:

Cites:

Information Journal Paper

Title

SSR MARKERS FOR SCREENING AND ESTIMATING GENETIC DISTANCE OF HOMOKARYOTIC SINGLE SPORES IN THE WHITE BUTTON MUSHROOM (AGARICUS BISPORUS)

Author(s)

BEYRATI M. | MALEKZADEH SHAFAROUDI S. | MALEKZADEH K. | MIRSHAMSI KAKHKI A. | Issue Writer Certificate

Keywords

WHITE BUTTON MUSHROOMQ2

HOMOKARYONQ2

HETEROKARYONQ2

MICROSATELLITE MARKERQ3

HYBRIDQ2

Abstract

Interspecies HYBRIDization is considered as the most important tools for improving genetic characteristics of Agaricus bisporus, which require having compatible HOMOKARYONs to cross. One challenge is low percentage of HOMOKARYONs among the basidiospores because of the secondary homothalism of this mushroom. Therefore, selection and confirmation of HOMOKARYONs among single spore isolates has always been involved with laborious efforts and researchers struggle with it. In this study, we attempted to develop a high-throughput method with high accuracy for screening and confirmation of HOMOKARYONs. We used 10 SSR markers -that represented nine linkage map groups of Agaricus bisporus-. Of 71 isolates, several isolates showed two bands as the same as those that heterokaryotic parents did, while some of them showed only one band. We were able to divide the isolates into two main groups: homoallelic and heteroallelic. The homoallelic group included 23 isolates that were monoallelic in the whole sites; which were thus confirmed as HOMOKARYONs. Further, the findings of the molecular markers were compared to the observations of a fruiting test. The results revealed that isolates that were confirmed by SSR to be heterokaryotic did not produce fruit bodies in the fruiting test. These findings obviously suggested that fruiting tests are less reliable than SSR markers. Genetic variation among 23 putative HOMOKARYONs was also calculated with the NTSYSpc software. The genetic similarity was variable between 0.3-1 among the isolates. The isolates were divided into two main groups by dendrogram. The results showed that these 10 SSR markers are able to detect HOMOKARYONs with a probability rate over 99 percent.

Cites

No record.

References

No record.

Cite

APA: Copy

BEYRATI, M., MALEKZADEH SHAFAROUDI, S., MALEKZADEH, K., & MIRSHAMSI KAKHKI, A.. (2015). SSR MARKERS FOR SCREENING AND ESTIMATING GENETIC DISTANCE OF HOMOKARYOTIC SINGLE SPORES IN THE WHITE BUTTON MUSHROOM (AGARICUS BISPORUS). JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 6(4), 23-36. SID. https://sid.ir/paper/224372/en

Vancouver: Copy

BEYRATI M., MALEKZADEH SHAFAROUDI S., MALEKZADEH K., MIRSHAMSI KAKHKI A.. SSR MARKERS FOR SCREENING AND ESTIMATING GENETIC DISTANCE OF HOMOKARYOTIC SINGLE SPORES IN THE WHITE BUTTON MUSHROOM (AGARICUS BISPORUS). JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2015;6(4):23-36. Available from: https://sid.ir/paper/224372/en

IEEE: Copy

M. BEYRATI, S. MALEKZADEH SHAFAROUDI, K. MALEKZADEH, and A. MIRSHAMSI KAKHKI, “SSR MARKERS FOR SCREENING AND ESTIMATING GENETIC DISTANCE OF HOMOKARYOTIC SINGLE SPORES IN THE WHITE BUTTON MUSHROOM (AGARICUS BISPORUS),” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 6, no. 4, pp. 23–36, 2015, [Online]. Available: https://sid.ir/paper/224372/en

Related Journal Papers