PHYLOGENETIC ANALYSIS AND SEQUENCING OF INCLUSION BODY GENE OF TWO IRANIAN ISOLATES OF CARNATION ETCHED RING VIRUS

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ASHNAYI M.; JAFARPOUR B.; MALEKZADEH SHAFAROUDI S.

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2014 | Volume:6 | Issue:2 Page(s): 1-14

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Title

PHYLOGENETIC ANALYSIS AND SEQUENCING OF INCLUSION BODY GENE OF TWO IRANIAN ISOLATES OF CARNATION ETCHED RING VIRUS

Author(s)

ASHNAYI M. | JAFARPOUR B. | MALEKZADEH SHAFAROUDI S. | Issue Writer Certificate

Keywords

PHYLOGENETIC ANALYSISQ1

POLYMERASE CHAIN REACTIONQ3

CARNATION ETCHED RING VIRUSQ2

Abstract

Carnation etched ring virus (CERV) is a member of family caulimoviridae, caulimovirus genus. The virus is the second important virus after Carnation mottle virus to infect carnation. In order to investigate the presence of this virus in greenhouses carnation plats of Khorasan Razavi (Mashhad) and Markazi (Mahallat) provinces samples which have been showed the symptoms were collected. Primary identification of CERV tested by Indirect enzyme-linked immunosorbent assay. Total DNA was extracted from fresh young leaf tissue by CTAB buffer. POLYMERASE CHAIN REACTION (PCR) was carried out using specific primers and fragments of 1500 bp were amplified by PCR. Comparisons were made between these isolates and related sequences of other caulimoviruses available in Genbank. The phylogenetic tree of inclusion body (IB) gene of CERV was drown, by MEGA5 software using Neighbor joining method. The results showed that the maximum similarity of Mahallat isolate was 99.4% to Indian isolate (AJ853858). Comparision of nucleotide sequences was shown the homology of two Iranian isolates together (92.2%) and with the other isolates (90-99.4%). Maximum amino acid changes were indicated in Holland isolate. Although there was a significant difference (60.6-92.9%) in amino acid level between the sequences of the IB region of different caulimovirus.

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APA: Copy

ASHNAYI, M., JAFARPOUR, B., & MALEKZADEH SHAFAROUDI, S.. (2014). PHYLOGENETIC ANALYSIS AND SEQUENCING OF INCLUSION BODY GENE OF TWO IRANIAN ISOLATES OF CARNATION ETCHED RING VIRUS. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 6(2), 1-14. SID. https://sid.ir/paper/224399/en

Vancouver: Copy

ASHNAYI M., JAFARPOUR B., MALEKZADEH SHAFAROUDI S.. PHYLOGENETIC ANALYSIS AND SEQUENCING OF INCLUSION BODY GENE OF TWO IRANIAN ISOLATES OF CARNATION ETCHED RING VIRUS. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2014;6(2):1-14. Available from: https://sid.ir/paper/224399/en

IEEE: Copy

M. ASHNAYI, B. JAFARPOUR, and S. MALEKZADEH SHAFAROUDI, “PHYLOGENETIC ANALYSIS AND SEQUENCING OF INCLUSION BODY GENE OF TWO IRANIAN ISOLATES OF CARNATION ETCHED RING VIRUS,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 6, no. 2, pp. 1–14, 2014, [Online]. Available: https://sid.ir/paper/224399/en

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