GENERATION OF ARABIDOPSIS EARLY FLOWERING PLANTS BY DESTRUCTION OF CLF TRANSCRIPTS VIA RNA SILENCING

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

DIDAR N.; PAZHOUHANDEH M.; MAHMOUDI F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2014 | Volume:6 | Issue:2 Page(s): 61-74

Download Full-Text

Persian Verion

View:

681

Download:

Cites:

Information Journal Paper

Title

GENERATION OF ARABIDOPSIS EARLY FLOWERING PLANTS BY DESTRUCTION OF CLF TRANSCRIPTS VIA RNA SILENCING

Author(s)

DIDAR N. | PAZHOUHANDEH M. | MAHMOUDI F. | Issue Writer Certificate

Keywords

ARABIDOPSIS THALIANAQ2

FLOWERINGQ2

CLF GENEQ2

EARLY FLOWERINGQ2

RNA SILENCINGQ2

Abstract

The control of FLOWERING time requires activation of a cascade of successive genes which are affected by several internal and external factors. Some of these genes, such as Curly Leaf (CLF), have inhibitory effect on FLOWERING. Thus, by silencing of these genes, initiation of FLOWERING can be accelerated. In this research, the RNA SILENCING technology is used to investigate the possibility of production of early-flowering plants via CLF silencing. First, in order to produce hairpin structure, a fragment of CLF GENE of ARABIDOPSIS THALIANA was amplified by PCR and then cloned in two sense and antisense directions, into pFGC5941 vector. The resulted recombinant vector was then transferred into Arabidopsis plants via Agrobacterium method. As expected, initiation of FLOWERING was accelerated in transgenic plants in comparison with the wild-type plants. In agreement to the obtained results, RT-qPCR analysis showed that CLF expression in transgenic plants was decreased in comparison with the wild-type plants. In contrast, FT (a gene which induces FLOWERING) expression was increased in transgenic plants. These results show that RNA SILENCING as a novel and time-saving biotechnological method could be applied to generate early-flowering plants.

Cites

No record.

References

No record.

Cite

APA: Copy

DIDAR, N., PAZHOUHANDEH, M., & MAHMOUDI, F.. (2014). GENERATION OF ARABIDOPSIS EARLY FLOWERING PLANTS BY DESTRUCTION OF CLF TRANSCRIPTS VIA RNA SILENCING. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 6(2), 61-74. SID. https://sid.ir/paper/224407/en

Vancouver: Copy

DIDAR N., PAZHOUHANDEH M., MAHMOUDI F.. GENERATION OF ARABIDOPSIS EARLY FLOWERING PLANTS BY DESTRUCTION OF CLF TRANSCRIPTS VIA RNA SILENCING. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2014;6(2):61-74. Available from: https://sid.ir/paper/224407/en

IEEE: Copy

N. DIDAR, M. PAZHOUHANDEH, and F. MAHMOUDI, “GENERATION OF ARABIDOPSIS EARLY FLOWERING PLANTS BY DESTRUCTION OF CLF TRANSCRIPTS VIA RNA SILENCING,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 6, no. 2, pp. 61–74, 2014, [Online]. Available: https://sid.ir/paper/224407/en

Related Journal Papers