POLYMORPHISMS IN FSHR AND GDF9 LOCI AND THEIR ASSOCIATIONS WITH LITTER SIZE IN ZEL SHEEP

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MORADI N.; NAZIFI N.; RAHIMI MIANJI G.; ANSARI PIRSARAEI Z.

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2014 | Volume:6 | Issue:2 Page(s): 163-176

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Title

POLYMORPHISMS IN FSHR AND GDF9 LOCI AND THEIR ASSOCIATIONS WITH LITTER SIZE IN ZEL SHEEP

Author(s)

MORADI N. | NAZIFI N. | RAHIMI MIANJI G. | ANSARI PIRSARAEI Z. | Issue Writer Certificate

Keywords

FSHRQ3

GDF9Q2

SSCPQ2

RFLPQ2

ZEL BREEDQ3

Abstract

Follicle stimulating hormone receptor (FSHR) and growth differentiation factor 9 (GDF9) both are the most important proteins that affect reproduction process in mammalian. Polymorphism in these genes and their possible relationship with twining trait in Zel (n=150) sheep are investigated in the present study. Blood samples were collected randomly via vein puncture. PCR-RFLP technique for FSHR marker site showed two A and B alleles with the frequency of 93.33 and 6.67 and two genotypes of AA and BB with the frequency of 93.33 and 6.67, respectively. However, the heterozygote AB genotype was not observed in studied samples. There was no significant association between FSHR marker site and twining (lambing per parity). Detection of genetic variation of GDF9 was carried out by PCR-RFLP and PCR-SSCP technique, respectively. All studied samples had similar banding pattern in both genetic markers and showed wild type homozygote AA genotype. Our finding in ZEL BREED in the present study along with pervious investigations on other major genes, It can be concluded that the common and major gene affecting reproduction trait are not present in Zel sheep. Therefore, further study is necessary to find a functional gene(s) in this breed.

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APA: Copy

MORADI, N., NAZIFI, N., RAHIMI MIANJI, G., & ANSARI PIRSARAEI, Z.. (2014). POLYMORPHISMS IN FSHR AND GDF9 LOCI AND THEIR ASSOCIATIONS WITH LITTER SIZE IN ZEL SHEEP. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 6(2), 163-176. SID. https://sid.ir/paper/224410/en

Vancouver: Copy

MORADI N., NAZIFI N., RAHIMI MIANJI G., ANSARI PIRSARAEI Z.. POLYMORPHISMS IN FSHR AND GDF9 LOCI AND THEIR ASSOCIATIONS WITH LITTER SIZE IN ZEL SHEEP. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2014;6(2):163-176. Available from: https://sid.ir/paper/224410/en

IEEE: Copy

N. MORADI, N. NAZIFI, G. RAHIMI MIANJI, and Z. ANSARI PIRSARAEI, “POLYMORPHISMS IN FSHR AND GDF9 LOCI AND THEIR ASSOCIATIONS WITH LITTER SIZE IN ZEL SHEEP,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 6, no. 2, pp. 163–176, 2014, [Online]. Available: https://sid.ir/paper/224410/en

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