ASSESSMENT OF DIVERSITY AND GENETIC RELATIONSHIPS IN POMEGRANATE GENOTYPES USING AFLP MARKER

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NAZIFI GELIRDI R.; KIANI G.; DEHESTANI KOLAGAR A.; HASHEMI S.H.R.

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2016 | Volume:8 | Issue:2 Page(s): 99-111

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Title

ASSESSMENT OF DIVERSITY AND GENETIC RELATIONSHIPS IN POMEGRANATE GENOTYPES USING AFLP MARKER

Author(s)

NAZIFI GELIRDI R. | KIANI G. | DEHESTANI KOLAGAR A. | HASHEMI S.H.R. | Issue Writer Certificate

Keywords

POMEGRANATEQ3

GENETIC DIVERSITYQ2

AFLP MARKERSQ2

PRINCIPAL COMPONENT ANALYSISQ2

Abstract

POMEGRANATE (Punica granatum L.) is native to Iran with several ecotypes which have been adapted to various climatic conditions and diseases. Although it is one of the important exported fruits, there is not a precise identification criteria to determine the authenticity of genetic inheritance and proprietary rights in the country. In the present study the genetic relationships of 47 POMEGRANATE genotypes, including 9 cultivated genotypes (from the collection of Yazd) and 38 wild genotypes (from Mazandaran, Gilan and Golestan provinces), were investigated with AFLP MARKERS. Using 10 primer combinations, 825 bands were obtained of which 697 showed polymorphism. Among the primers, primer combination of M-CTT and E-ACG showed the highest number of bands (148) and primer combination of E-ACC, M-CTG produced the lowest number of bands (40). The average value for PIC was 0.21, while Average Marker Index (MI) was 17.33 that the highest one belonged to primer combination of M-CAC and E-AAG. Maximum genetic similarity belonged to Bahnamir with Shirin Kan Tehran samples and the minimum genetic similarity observed between Berenjestanak and Khosh Darreh. PRINCIPAL COMPONENT ANALYSIS showed that 57% of the total variance was explained by first three components. The identified primer combinations in this study could be used as a powerful tool for determination of genetic relationships between POMEGRANATE genotypes.

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APA: Copy

NAZIFI GELIRDI, R., KIANI, G., DEHESTANI KOLAGAR, A., & HASHEMI, S.H.R.. (2016). ASSESSMENT OF DIVERSITY AND GENETIC RELATIONSHIPS IN POMEGRANATE GENOTYPES USING AFLP MARKER. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 8(2), 99-111. SID. https://sid.ir/paper/224516/en

Vancouver: Copy

NAZIFI GELIRDI R., KIANI G., DEHESTANI KOLAGAR A., HASHEMI S.H.R.. ASSESSMENT OF DIVERSITY AND GENETIC RELATIONSHIPS IN POMEGRANATE GENOTYPES USING AFLP MARKER. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2016;8(2):99-111. Available from: https://sid.ir/paper/224516/en

IEEE: Copy

R. NAZIFI GELIRDI, G. KIANI, A. DEHESTANI KOLAGAR, and S.H.R. HASHEMI, “ASSESSMENT OF DIVERSITY AND GENETIC RELATIONSHIPS IN POMEGRANATE GENOTYPES USING AFLP MARKER,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 8, no. 2, pp. 99–111, 2016, [Online]. Available: https://sid.ir/paper/224516/en

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