EVALUATION OF IL-2 AND IL-12 MRNA EXPRESSION IN VACCINATED GUINEA PIGS WITH INACTIVATED TYPE O FOOT-AND-MOUTH DISEASE VACCINE

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HONARBAKHSH E.; ASLAMINEJAD A.S.; NASSIRI M.R.; ZIBAEI S.; PASANDIDEH R.

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2016 | Volume:8 | Issue:2 Page(s): 113-126

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Title

EVALUATION OF IL-2 AND IL-12 MRNA EXPRESSION IN VACCINATED GUINEA PIGS WITH INACTIVATED TYPE O FOOT-AND-MOUTH DISEASE VACCINE

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Keywords

FOOT-AND-MOUTH DISEASE (FMD)Q2

GUINEA PIGQ1

IL-2Q1

IL-12Q1

REAL-TIME PCRQ1

Abstract

Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofed livestock and wildlife. Foot-and-mouth disease is an endemic disease in Iran that sometimes caused considerable losses of livestock, especially in young animals. In this study mRNA expression of interleukin-2 (IL-2) and interleukin-12 (IL-12) cytokines was evaluated in vaccinated GUINEA PIGs with inactivated type O FMD vaccine (without adjuvant). Blood samples were collected from saphenous vein of eight male GUINEA PIGs (at control and vaccinated groups) at 7 and 28 days after the first vaccination. Total mRNAs were extracted using the column RNA isolation kit and subsequently reverse-transcribed into cDNA. Then relative REAL-TIME PCR assay was used to analyze of quantification of IL-2 and IL-12 expression. The expression of IL-2 gene had not significant difference in vaccinated animals compared to the control group in the first and second blood samples but IL-12 gene expression significantly increased (P<0.05). Evaluation of cytokines genes expression can be as a valuable adjunct for assessment of effects of new vaccines on immune system in future studies.

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APA: Copy

HONARBAKHSH, E., ASLAMINEJAD, A.S., NASSIRI, M.R., ZIBAEI, S., & PASANDIDEH, R.. (2016). EVALUATION OF IL-2 AND IL-12 MRNA EXPRESSION IN VACCINATED GUINEA PIGS WITH INACTIVATED TYPE O FOOT-AND-MOUTH DISEASE VACCINE. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 8(2), 113-126. SID. https://sid.ir/paper/224517/en

Vancouver: Copy

HONARBAKHSH E., ASLAMINEJAD A.S., NASSIRI M.R., ZIBAEI S., PASANDIDEH R.. EVALUATION OF IL-2 AND IL-12 MRNA EXPRESSION IN VACCINATED GUINEA PIGS WITH INACTIVATED TYPE O FOOT-AND-MOUTH DISEASE VACCINE. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2016;8(2):113-126. Available from: https://sid.ir/paper/224517/en

IEEE: Copy

E. HONARBAKHSH, A.S. ASLAMINEJAD, M.R. NASSIRI, S. ZIBAEI, and R. PASANDIDEH, “EVALUATION OF IL-2 AND IL-12 MRNA EXPRESSION IN VACCINATED GUINEA PIGS WITH INACTIVATED TYPE O FOOT-AND-MOUTH DISEASE VACCINE,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 8, no. 2, pp. 113–126, 2016, [Online]. Available: https://sid.ir/paper/224517/en

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