ASSESSMENT OF GENETIC DIVERSITY IN IRANIAN CONFECTIONARY SUNFLOWER (HELIANTHUS ANNUUS L.) POPULATIONS USING RETROTRANSPOSON BASED IRAP MARKERS

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BASIRNIA A.; DARVISHZADEH R.; ABDOLLAHI MANDOULAKANI B.; NABIPUR A.

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Paper Information

Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2014 | Volume:6 | Issue:1 Page(s): 19-33

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Title

ASSESSMENT OF GENETIC DIVERSITY IN IRANIAN CONFECTIONARY SUNFLOWER (HELIANTHUS ANNUUS L.) POPULATIONS USING RETROTRANSPOSON BASED IRAP MARKERS

Author(s)

BASIRNIA A. | DARVISHZADEH R. | ABDOLLAHI MANDOULAKANI B. | NABIPUR A. | Issue Writer Certificate

Keywords

CLUSTER ANALYSISQ2

LANDRACEQ2

RETRO TRANSPOSONQ2

SUNFLOWERQ2

MOLECULAR MARKERQ2

Abstract

Genetic diversity is necessary for plant breeders to obtain new cultivars either with high yield, better quality, more adapted to abiotic stress or more resistant to pest and pathogens. RETRO TRANSPOSONs are current component of plant genomes. Ubiquitous, activity and abundant of RETRO TRANSPOSONs thorough the plant genome, make them useful MOLECULAR MARKER. We used inter-retro transposon amplified polymorphism (IRAP) markers to assess genomic diversity levels and survey the activity of LTR retrotransposon elements in 10 confectionary SUNFLOWER populations. Out of 25 single and combined IRAP primers, 11 primers produced scorable and polymorphic banding patterns. A total of 116 loci were amplified by using 11 IRAP primers on confectionary populations.110 loci out of 116 were polymorphic. The lowest (0.74) and highest (0.88) Nei genetic similarity was observed between Hamedan and Mashhad and Marand and Esfahan populations, respectively. CLUSTER ANALYSIS based on UPGMA algorithm grouped the studied populations in 3 main classes.Analysis of molecular variance revealed that the high part of total variation was due to within populations. So it will be better to do selection within populations in breeding programs. The results showed that RETRO TRANSPOSONs are active in SUNFLOWER genome and they are inserted in SUNFLOWER genome as head-to-head, head-to-tail and tail-to-tail orientations. Our results showed that RETRO TRANSPOSON-based MOLECULAR MARKERs can be used as a valuable tool for genomic assessment in SUNFLOWER.

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APA: Copy

BASIRNIA, A., DARVISHZADEH, R., ABDOLLAHI MANDOULAKANI, B., & NABIPUR, A.. (2014). ASSESSMENT OF GENETIC DIVERSITY IN IRANIAN CONFECTIONARY SUNFLOWER (HELIANTHUS ANNUUS L.) POPULATIONS USING RETROTRANSPOSON BASED IRAP MARKERS. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 6(1), 19-33. SID. https://sid.ir/paper/224540/en

Vancouver: Copy

BASIRNIA A., DARVISHZADEH R., ABDOLLAHI MANDOULAKANI B., NABIPUR A.. ASSESSMENT OF GENETIC DIVERSITY IN IRANIAN CONFECTIONARY SUNFLOWER (HELIANTHUS ANNUUS L.) POPULATIONS USING RETROTRANSPOSON BASED IRAP MARKERS. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2014;6(1):19-33. Available from: https://sid.ir/paper/224540/en

IEEE: Copy

A. BASIRNIA, R. DARVISHZADEH, B. ABDOLLAHI MANDOULAKANI, and A. NABIPUR, “ASSESSMENT OF GENETIC DIVERSITY IN IRANIAN CONFECTIONARY SUNFLOWER (HELIANTHUS ANNUUS L.) POPULATIONS USING RETROTRANSPOSON BASED IRAP MARKERS,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 6, no. 1, pp. 19–33, 2014, [Online]. Available: https://sid.ir/paper/224540/en

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