ISOLATION, GENE EXPRESSION AND PTHA EFFECTOR PROTEIN PRODUCTION OF XANTHOMONAS CITRI SUBSP CITRI CAUSAL AGENT OF CITRUS BACTERIAL CANKER

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MOKHTARI M.; SAFARNEJAD M.R.; ALAVI S.M.; TORKAMANZEHI A.

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Paper Information

Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2015 | Volume:7 | Issue:2 Page(s): 155-170

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Title

ISOLATION, GENE EXPRESSION AND PTHA EFFECTOR PROTEIN PRODUCTION OF XANTHOMONAS CITRI SUBSP CITRI CAUSAL AGENT OF CITRUS BACTERIAL CANKER

Author(s)

MOKHTARI M. | SAFARNEJAD M.R. | ALAVI S.M. | TORKAMANZEHI A. | Issue Writer Certificate

Keywords

CITRUS BACTERIAL CANKERQ2

XANTHOMONAS CITRI SUBSP CITRIQ2

RECOMBINANT PROTEINQ2

PTHAQ2

EFFECTOR PROTEINQ2

Abstract

The CITRUS BACTERIAL CANKER is among the most important diseases in Mexican lime gardens in southern area of Iran. The disease is caused byXanthomonas citri subsp. citri (Xcc). The PTHA bacterial protein, as an EFFECTOR PROTEIN, is crucial in pathogenesis pathway.Inhibition of its functions through antibody could lead to suppression of disease in plant.The present study was performed for isolation and expression ofpthA gene and purification of PTHA RECOMBINANT PROTEIN. For this aim the specific primers were designed for isolation of 606 bp of hydroxyl terminal of this gene followed by PCR amplification and cloning into pTZ57R/T vector. The coding sequence of truncated PTHA was inserted to pET28a bacterial expression vector as a C-terminal fusion to 6XHis tag. Production of RECOMBINANT PROTEIN was performed in BL21 (DE3) strain ofE. coli. The purification was done under native condition through affinity chromatography on nickel column. Total yield was assessed by SDS-PAGE and western blotting analysis. The results confirmed production of PTHA RECOMBINANT PROTEIN with molecular weight around 26 kDa. The purified RECOMBINANT PROTEIN could be used as an antigen for production of recombinant monoclonal antibodies.

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APA: Copy

MOKHTARI, M., SAFARNEJAD, M.R., ALAVI, S.M., & TORKAMANZEHI, A.. (2015). ISOLATION, GENE EXPRESSION AND PTHA EFFECTOR PROTEIN PRODUCTION OF XANTHOMONAS CITRI SUBSP CITRI CAUSAL AGENT OF CITRUS BACTERIAL CANKER. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 7(2), 155-170. SID. https://sid.ir/paper/224575/en

Vancouver: Copy

MOKHTARI M., SAFARNEJAD M.R., ALAVI S.M., TORKAMANZEHI A.. ISOLATION, GENE EXPRESSION AND PTHA EFFECTOR PROTEIN PRODUCTION OF XANTHOMONAS CITRI SUBSP CITRI CAUSAL AGENT OF CITRUS BACTERIAL CANKER. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2015;7(2):155-170. Available from: https://sid.ir/paper/224575/en

IEEE: Copy

M. MOKHTARI, M.R. SAFARNEJAD, S.M. ALAVI, and A. TORKAMANZEHI, “ISOLATION, GENE EXPRESSION AND PTHA EFFECTOR PROTEIN PRODUCTION OF XANTHOMONAS CITRI SUBSP CITRI CAUSAL AGENT OF CITRUS BACTERIAL CANKER,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 7, no. 2, pp. 155–170, 2015, [Online]. Available: https://sid.ir/paper/224575/en

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