ISOLATION, CLONING AND MOLECULAR CHARACTERIZATION OF GENES ENCODING SINGLE-STRAND SPECIFIC ENDONUCLEASES FROM PARSLEY (PETROSELINUM CRISPUM L.)

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MIRZAEI M.; ZOLALA J.; SHALOUZAD A.

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2015 | Volume:7 | Issue:1 Page(s): 201-215

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Title

ISOLATION, CLONING AND MOLECULAR CHARACTERIZATION OF GENES ENCODING SINGLE-STRAND SPECIFIC ENDONUCLEASES FROM PARSLEY (PETROSELINUM CRISPUM L.)

Author(s)

MIRZAEI M. | ZOLALA J. | SHALOUZAD A. | Issue Writer Certificate

Keywords

SINGLE-STRAND SPECIFIC NUCLEASESQ2

PETROSELINUM CRISPUMQ2

ENZYMATIC CLEAVAGE OF HETERODUPLEX DNAQ2

CLONINGQ2

Abstract

Single-strand specific (sss) nucleases (S1/P1 family) are widely used in molecular genetics studies. The most widely used sss-nucleases are those plant endonucleases with the ability of specific cleavage of mismatch loops in heteroduplex DNA molecules. Because of the valuable activity of these endonucleases, ENZYMATIC CLEAVAGE OF HETERODUPLEX DNA molecules is one of the most common techniques of point mutation detection in genomes. In this study, gene sequences encoding single-strand specific endonucleases were isolated from parsley (Petroselinum crispum L.) using heterologous primers in PCR reactions. The isolated gene sequences, after CLONING and molecular identification, were designated as pars I and pars II. Physicochemical, structural and phylogenetic analysis using software tools, verified PARS I and PARS II as two members of S1/P1 family with a substantial homology to heteroduplex DNA specific endonucleases. However, it is essential to produce pure enzyme preparations as native or recombinant forms to determine mechanisms of activity and substrate preferences of these nucleases on heteroduplex DNA substrates.

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APA: Copy

MIRZAEI, M., ZOLALA, J., & SHALOUZAD, A.. (2015). ISOLATION, CLONING AND MOLECULAR CHARACTERIZATION OF GENES ENCODING SINGLE-STRAND SPECIFIC ENDONUCLEASES FROM PARSLEY (PETROSELINUM CRISPUM L.). JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 7(1), 201-215. SID. https://sid.ir/paper/224586/en

Vancouver: Copy

MIRZAEI M., ZOLALA J., SHALOUZAD A.. ISOLATION, CLONING AND MOLECULAR CHARACTERIZATION OF GENES ENCODING SINGLE-STRAND SPECIFIC ENDONUCLEASES FROM PARSLEY (PETROSELINUM CRISPUM L.). JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2015;7(1):201-215. Available from: https://sid.ir/paper/224586/en

IEEE: Copy

M. MIRZAEI, J. ZOLALA, and A. SHALOUZAD, “ISOLATION, CLONING AND MOLECULAR CHARACTERIZATION OF GENES ENCODING SINGLE-STRAND SPECIFIC ENDONUCLEASES FROM PARSLEY (PETROSELINUM CRISPUM L.),” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 7, no. 1, pp. 201–215, 2015, [Online]. Available: https://sid.ir/paper/224586/en

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