SEQUENCING OF THIRD EXON OF IGF1 GENE IN SHEEP

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SAJADI ZARJANI S.; BAHREINI BEHZADI M.R.; FARDAEI M.

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Paper Information

Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2016 | Volume:7 | Issue:4 Page(s): 83-84

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Title

SEQUENCING OF THIRD EXON OF IGF1 GENE IN SHEEP

Author(s)

SAJADI ZARJANI S. | BAHREINI BEHZADI M.R. | FARDAEI M. | Issue Writer Certificate

Keywords

SEQUENCINGQ1

POLYMORPHISMQ2

IGF1 GENEQ2

PROTEIN STRUCTUREQ1

SHEEPQ3

Abstract

Insulin-like growth factor is a single-chain polypeptide. The Molecular weight of it is 7.5 KDa. This factor is insulin like activity and the most important growth factor in the body and plays important role in the proliferation, differentiation and cells growth. IGF1 GENE located on chromosome 3 in SHEEP. This gene i s more proposed as a candida gene for growth and meat quality traits in animal genetic improvement programs. The aim of this research is to find POLYMORPHISMs in the third exon. In this research blood samples of breeds of 25 SHEEP include Mehraban, Karakul, Ghezel, Lak Ghashghaei and Bahmaei were collected with EDTA tube. The DNA was extracted from blood samples and the third exon region wa s amplified with specific primers using PCR and SEQUENCING. The results showed, there is 2 SNP in intron 2 and 3. Protein structure of IGF1 GENE is investigated by using Swiss-pdb viewer software. PROTEIN STRUCTURE analysis showed that this protein is highly conserved of e volution. This protein has a structure consisting of an alpha helix with irregular structure.

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APA: Copy

SAJADI ZARJANI, S., BAHREINI BEHZADI, M.R., & FARDAEI, M.. (2016). SEQUENCING OF THIRD EXON OF IGF1 GENE IN SHEEP. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 7(4), 83-84. SID. https://sid.ir/paper/224601/en

Vancouver: Copy

SAJADI ZARJANI S., BAHREINI BEHZADI M.R., FARDAEI M.. SEQUENCING OF THIRD EXON OF IGF1 GENE IN SHEEP. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2016;7(4):83-84. Available from: https://sid.ir/paper/224601/en

IEEE: Copy

S. SAJADI ZARJANI, M.R. BAHREINI BEHZADI, and M. FARDAEI, “SEQUENCING OF THIRD EXON OF IGF1 GENE IN SHEEP,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 7, no. 4, pp. 83–84, 2016, [Online]. Available: https://sid.ir/paper/224601/en

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