DIRECT EXTRACTION AND PURIFICATION OF SOIL MICROBIAL TOTAL DNA AND MOLECULAR DETECTION OF SOIL-BORNE PATHOGEN FUSARIUM OXYSPORUM CAUSAL AGENT OF TOMATO VASCULAR WILT

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EBRAHIMI MOHAMMAD ALI; ALAHYAR PARSA SHIVA; PIRAISH SAEEDEH

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Journal: Crop Biotechnology Year:2016 | Volume:4 | Issue:12 Page(s): 55-66

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Title

DIRECT EXTRACTION AND PURIFICATION OF SOIL MICROBIAL TOTAL DNA AND MOLECULAR DETECTION OF SOIL-BORNE PATHOGEN FUSARIUM OXYSPORUM CAUSAL AGENT OF TOMATO VASCULAR WILT

Author(s)

EBRAHIMI MOHAMMAD ALI | ALAHYAR PARSA SHIVA | PIRAISH SAEEDEH | Issue Writer Certificate

Keywords

DNA EXTRACTIONQ2

MOLECULAR TRACKQ2

FUSARIUM OXYSPORUMF. SP LYCOPERSICIQ2

FUSARIUM OXYSPORUMF. SP. RADICIS LYCOPERSICIQ2

Abstract

Molecular analysis is often required in many genome extracted from various tissues. In much molecular analysis of genetic studies of microbial communities, often require direct extraction of DNA from soil. In this study, after careful study of the various methods proposed three methods (chemical enzyme), (chemimechanical) and (chemical mechanical enzymatic), in order to extract DNA from soil, a separate project was studied to most identify different methods of extraction. The comparison between the same conditions, a significant difference in the DNA product of the average concentration of 2.32 to 6.1 ng per μl DNA was diluted and also on the basis of information obtained from Nanodrop and electrophoresis device and review of the DNA extracted protein product DNA, methods (chemical, mechanical, enzymatic) better performance at higher concentrations, remove humic acid proteins and other contaminants was superior to other methods. The molecular detection of pathogens that extraction is important applications; the aim of this study is molecular detection of pathogenFusarium oxysporumf. sp lycopersici and Fusarium oxysporum f. sp. radicis lycopersic i of soil microbial community by molecular techniques to identify the special primer evaluated. Molecular detection results showed that there is tomato Fusarium infection in soil samples S1.

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APA: Copy

EBRAHIMI, MOHAMMAD ALI, ALAHYAR PARSA, SHIVA, & PIRAISH, SAEEDEH. (2016). DIRECT EXTRACTION AND PURIFICATION OF SOIL MICROBIAL TOTAL DNA AND MOLECULAR DETECTION OF SOIL-BORNE PATHOGEN FUSARIUM OXYSPORUM CAUSAL AGENT OF TOMATO VASCULAR WILT. JOURNAL OF CROP BIOTECHNOLOGY, 4(12), 55-66. SID. https://sid.ir/paper/226778/en

Vancouver: Copy

EBRAHIMI MOHAMMAD ALI, ALAHYAR PARSA SHIVA, PIRAISH SAEEDEH. DIRECT EXTRACTION AND PURIFICATION OF SOIL MICROBIAL TOTAL DNA AND MOLECULAR DETECTION OF SOIL-BORNE PATHOGEN FUSARIUM OXYSPORUM CAUSAL AGENT OF TOMATO VASCULAR WILT. JOURNAL OF CROP BIOTECHNOLOGY[Internet]. 2016;4(12):55-66. Available from: https://sid.ir/paper/226778/en

IEEE: Copy

MOHAMMAD ALI EBRAHIMI, SHIVA ALAHYAR PARSA, and SAEEDEH PIRAISH, “DIRECT EXTRACTION AND PURIFICATION OF SOIL MICROBIAL TOTAL DNA AND MOLECULAR DETECTION OF SOIL-BORNE PATHOGEN FUSARIUM OXYSPORUM CAUSAL AGENT OF TOMATO VASCULAR WILT,” JOURNAL OF CROP BIOTECHNOLOGY, vol. 4, no. 12, pp. 55–66, 2016, [Online]. Available: https://sid.ir/paper/226778/en

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