ISOLATION AND IDENTIFICATION OF DOMINANT ARBUSCULAR MYCORRHIZAL FUNGI IN THE RHIZOSPHERE OF WHEAT, BARLEY AND WEEDS IN SOME SALINE REGIONS OF IRAN

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SALEHI JOUZANI GH.; AKBARI VALA S.; SABET JAHROMI M.; MORSALI H.

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Journal: Crop Biotechnology Year:2011 | Volume:1 | Issue:1 Page(s): 61-75

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Title

ISOLATION AND IDENTIFICATION OF DOMINANT ARBUSCULAR MYCORRHIZAL FUNGI IN THE RHIZOSPHERE OF WHEAT, BARLEY AND WEEDS IN SOME SALINE REGIONS OF IRAN

Author(s)

SALEHI JOUZANI GH. | AKBARI VALA S. | SABET JAHROMI M. | MORSALI H. | Issue Writer Certificate

Keywords

ARBUSCULAR MYCORRHIZAL FUNGI (AMF)Q2

SALINITY TOLERANCEQ2

MOLECULAR IDENTIFICATIONQ2

INTERNAL TRANSCRIBED SPACER (ITS)Q2

NESTED PCRQ2

Abstract

Commonly, plant roots colonized by arbuscular mycorrhizal fungi (AMF) can tolerate different stresses such as soil salinity. Thereby, identification of the dominant AMF species in the saline soils and their application as biofertilizer could be very useful in order to increase crop productivity under such conditions. For this purpose, sampling was performed from root and rhizosphere of wheat, barley and weeds in Yazd, East Azerbaijan, Qom and Markazi provinces of Iran. The spore morphological properties of the isolated AMFs were studied. Then, samples were screened using a two-step NESTED PCR methodology. At the first step, AMF-specific primers, including LSU-Glom1 and SSU- Glom1 were used, followed by Alu1 digestion of the PCR products, and then at the second step, the digested PCR products were amplified by using fungal universal primers (ITS4 and ITS5) in order to amplify the ITS-rDNA region. The PCR products were then cloned, and digested by Taq1. The results of the morphological characteristics and sequence analyses showed that two AMF genus, including Glomus (more than 90%) and Acaulospora (10%) were dominant. The species G. mosseae (50%), G. intraradices, G. sinosum, G. constrictum, G. etunicatum, G. versiforme, G. fulvom, and Glomus sp were identified using the molecular analysis. The maximum species diversity was observed in the fields located in Yazd Province and in rhizosphere of wheat. Overall, the results of the present study showed that the species G. mosseae had the highest dominancy and adaptivity under saline conditions. Hence, this species can be used as a source of biofertilizer in such regions after performing complementary experiments.

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APA: Copy

SALEHI JOUZANI, GH., AKBARI VALA, S., SABET JAHROMI, M., & MORSALI, H.. (2011). ISOLATION AND IDENTIFICATION OF DOMINANT ARBUSCULAR MYCORRHIZAL FUNGI IN THE RHIZOSPHERE OF WHEAT, BARLEY AND WEEDS IN SOME SALINE REGIONS OF IRAN. JOURNAL OF CROP BIOTECHNOLOGY, 1(1), 61-75. SID. https://sid.ir/paper/226791/en

Vancouver: Copy

SALEHI JOUZANI GH., AKBARI VALA S., SABET JAHROMI M., MORSALI H.. ISOLATION AND IDENTIFICATION OF DOMINANT ARBUSCULAR MYCORRHIZAL FUNGI IN THE RHIZOSPHERE OF WHEAT, BARLEY AND WEEDS IN SOME SALINE REGIONS OF IRAN. JOURNAL OF CROP BIOTECHNOLOGY[Internet]. 2011;1(1):61-75. Available from: https://sid.ir/paper/226791/en

IEEE: Copy

GH. SALEHI JOUZANI, S. AKBARI VALA, M. SABET JAHROMI, and H. MORSALI, “ISOLATION AND IDENTIFICATION OF DOMINANT ARBUSCULAR MYCORRHIZAL FUNGI IN THE RHIZOSPHERE OF WHEAT, BARLEY AND WEEDS IN SOME SALINE REGIONS OF IRAN,” JOURNAL OF CROP BIOTECHNOLOGY, vol. 1, no. 1, pp. 61–75, 2011, [Online]. Available: https://sid.ir/paper/226791/en

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