DETERMINATION OF FUNCTIONAL DOMAINS AND TOPOLOGY MODEL OF MEMBRANE-BOUND DELTA-6 DESATURASE ENZYME FROM MORTIERELLA ALPINE

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ASHAAR GHADIM ESMAT; GHARANJIK SHAHROKH; BAGHBAN KOHNEROUZ BAHRAM

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Journal: Crop Biotechnology Year:2016 | Volume:6 | Issue:15 Page(s): 13-23

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Title

DETERMINATION OF FUNCTIONAL DOMAINS AND TOPOLOGY MODEL OF MEMBRANE-BOUND DELTA-6 DESATURASE ENZYME FROM MORTIERELLA ALPINE

Author(s)

ASHAAR GHADIM ESMAT | GHARANJIK SHAHROKH | BAGHBAN KOHNEROUZ BAHRAM | Issue Writer Certificate

Keywords

Δ6 DESATURASES ENZYMEQ1

MEMBRANEBOUND DESATURASEQ1

DOMAINQ1

MORTIERELLA ALPINAQ1

Abstract

Desaturase enzymes (EC: 1.14.19.3) catalyze dehydrogenation reactions and creates a double bond in the fatty acid chains. These enzymes classified into two groups, soluble and membrane each with different consensus motifs. Despite the Delta-6 desaturase is a key enzyme in the biosynthesis of unsaturated fatty acids but due to lack of structural information of membrane-bound desaturase, it's three-dimensional and crystal structure has not been determined. MORTIERELLA ALPINA is a rich source for production of unsaturated fatty acid arachidonic and has an active Delta-6 desaturases enzyme. The aim of this study was obtain encoding sequences of Delta-6 desaturase gene, and determination of functional DOMAINs using comparative analysis and molecular model and propose a membrane topology model for this enzyme. Thus, after extraction of total RNA and cDNA synthesis using gene specific primers, PCR product was cloned into pBlueScriptSK+ vector and then sequenced. Based on bioinformatics analysis by Swissmodel, Predict and Phobius servers presence of functional DOMAINs cytochrome b5 carrying motif (HPGG), three His-box motifs and transmembrane regions were identified. Then we proposed a membrane topology model for the Delta-6 desaturase enzyme.

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APA: Copy

ASHAAR GHADIM, ESMAT, GHARANJIK, SHAHROKH, & BAGHBAN KOHNEROUZ, BAHRAM. (2016). DETERMINATION OF FUNCTIONAL DOMAINS AND TOPOLOGY MODEL OF MEMBRANE-BOUND DELTA-6 DESATURASE ENZYME FROM MORTIERELLA ALPINE. JOURNAL OF CROP BIOTECHNOLOGY, 6(15), 13-23. SID. https://sid.ir/paper/226798/en

Vancouver: Copy

ASHAAR GHADIM ESMAT, GHARANJIK SHAHROKH, BAGHBAN KOHNEROUZ BAHRAM. DETERMINATION OF FUNCTIONAL DOMAINS AND TOPOLOGY MODEL OF MEMBRANE-BOUND DELTA-6 DESATURASE ENZYME FROM MORTIERELLA ALPINE. JOURNAL OF CROP BIOTECHNOLOGY[Internet]. 2016;6(15):13-23. Available from: https://sid.ir/paper/226798/en

IEEE: Copy

ESMAT ASHAAR GHADIM, SHAHROKH GHARANJIK, and BAHRAM BAGHBAN KOHNEROUZ, “DETERMINATION OF FUNCTIONAL DOMAINS AND TOPOLOGY MODEL OF MEMBRANE-BOUND DELTA-6 DESATURASE ENZYME FROM MORTIERELLA ALPINE,” JOURNAL OF CROP BIOTECHNOLOGY, vol. 6, no. 15, pp. 13–23, 2016, [Online]. Available: https://sid.ir/paper/226798/en

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