Detection of Mycotoxins Producing of Fusarium Species Using Morphological and Molecular Characteristics in Corn

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Javanbakht Avval N.; Moradzadeh Esakandari M.; AFZALI H.; OKHOVVAT S.M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Applied Research in Plant Protection Year:2018 | Volume:7 | Issue:1 Page(s): 1-16

Download Full-Text

Persian Verion

View:

638

Download:

Cites:

Information Journal Paper

Title

Detection of Mycotoxins Producing of Fusarium Species Using Morphological and Molecular Characteristics in Corn

Author(s)

Javanbakht Avval N. | Moradzadeh Esakandari M. | AFZALI H. | OKHOVVAT S.M. | Issue Writer Certificate

Keywords

Prevalent speciesQ1

Fusarium verticillioidsQ1

FumonisinQ1

Specific PrimerQ1

Abstract

Fusarium, a phytopathogenic fungus in fields of corn, not only reduces the quality and quantity of crops but also causes food contamination and endangers people ̓ s health by producing some mycotoxins such as Fumonisin (FUM). In the present study, samples were collected from fungal disease symptoms-showing corn from different livestock and poultry storage in Khorasan-Razavi province. Having placed the corn on PDA and WA, isolates belonging to Fusarium genus were isolated and purified. As a result, a total of 40 isolates were obtained among which three species were identified as Fusarium verticillioides, F. graminearum and F. proliferatum with isolated frequencies of 34, 4 and 2, respectively. To support these results, DNA was extracted from the isolates from corn seeds and the fungal ribosomal DNA was amplified using PCR with species-Specific Primers. In addition, using PCR with the Specific Primers (FUM1 F/R) 15 isolates of F. verticillioides, as the Prevalent species were examined for Fumonisin production. The results indicated that all of the examined F. verticllioides isolates gave the expected fragment with 183 bp in size. Therefore, this study demonstrated that F. verticllioides isolates genetically have a potential for Fumonisin-production. It also showed that the PCR-based technique can be used for the identification, distinction and detection of the Fusarium species.

Cites

No record.

References

No record.

Cite

APA: Copy

Javanbakht Avval, N., Moradzadeh Esakandari, M., AFZALI, H., & OKHOVVAT, S.M.. (2018). Detection of Mycotoxins Producing of Fusarium Species Using Morphological and Molecular Characteristics in Corn. JOURNAL OF APPLIED RESEARCHES IN PLANT PROTECTION, 7(1 ), 1-16. SID. https://sid.ir/paper/237588/en

Vancouver: Copy

Javanbakht Avval N., Moradzadeh Esakandari M., AFZALI H., OKHOVVAT S.M.. Detection of Mycotoxins Producing of Fusarium Species Using Morphological and Molecular Characteristics in Corn. JOURNAL OF APPLIED RESEARCHES IN PLANT PROTECTION[Internet]. 2018;7(1 ):1-16. Available from: https://sid.ir/paper/237588/en

IEEE: Copy

N. Javanbakht Avval, M. Moradzadeh Esakandari, H. AFZALI, and S.M. OKHOVVAT, “Detection of Mycotoxins Producing of Fusarium Species Using Morphological and Molecular Characteristics in Corn,” JOURNAL OF APPLIED RESEARCHES IN PLANT PROTECTION, vol. 7, no. 1 , pp. 1–16, 2018, [Online]. Available: https://sid.ir/paper/237588/en

Related Journal Papers