Characterization and genetic variability of coat protein gene of Apple chlorotic leaf spot virus isolates from apple in Kurdistan and western Azarbaijan Provinces, Iran

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Shujaei Kamal; Azaryar Mahdi; HAJIZADEH MOHAMMAD

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Journal: JOURNAL OF CROP PROTECTION Year:2019 | Volume:8 | Issue:4 Page(s): 491-500

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Title

Characterization and genetic variability of coat protein gene of Apple chlorotic leaf spot virus isolates from apple in Kurdistan and western Azarbaijan Provinces, Iran

Author(s)

Shujaei Kamal | Azaryar Mahdi | HAJIZADEH MOHAMMAD | Issue Writer Certificate

Keywords

appleQ2

negative selectionQ1

phylogenetic analysisQ1

ACLSVQ1

Abstract

In this study, 174 leaf samples from main apple growing areas in Kurdistan and western Azarbaijan Provinces were examined for infection by apple chlorotic leaf spot virus (ACLSV). The result of RT-PCR showed that 17 samples (9. 7%) were infected by ACLSV in both provinces. Subsequently, the genetic structure of ACLSV was determined based on the full coat protein (CP) sequences of eight isolates from this study and those recently published from Iran and worldwide. In phylogenetic analysis, ACLSV isolates from apple clustered into three known phylogenetic types; B6, P205, and SHZ, which are characterized by three amino acids at positions of 40, 75 and 79. The new Iranian ACLSV isolates showed the CP amino acid sequence of Ser40-Tyr75-Glu79 which placed them with the B6 type. Further analyses showed that the Iranian ACLSV isolates (the new and previously published isolates) had lower genetic variability compared to isolates from other countries except for Brazilian population. Most of the CP codons were under negative selection (dN/dS < 1) except for one codon at position 92 which was under positive selection. This study suggests that negative selection was most important evolutionary factor driving the genetic evolution of ACLSV.

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APA: Copy

Shujaei, Kamal, Azaryar, Mahdi, & HAJIZADEH, MOHAMMAD. (2019). Characterization and genetic variability of coat protein gene of Apple chlorotic leaf spot virus isolates from apple in Kurdistan and western Azarbaijan Provinces, Iran. JOURNAL OF CROP PROTECTION, 8(4 ), 491-500. SID. https://sid.ir/paper/238923/en

Vancouver: Copy

Shujaei Kamal, Azaryar Mahdi, HAJIZADEH MOHAMMAD. Characterization and genetic variability of coat protein gene of Apple chlorotic leaf spot virus isolates from apple in Kurdistan and western Azarbaijan Provinces, Iran. JOURNAL OF CROP PROTECTION[Internet]. 2019;8(4 ):491-500. Available from: https://sid.ir/paper/238923/en

IEEE: Copy

Kamal Shujaei, Mahdi Azaryar, and MOHAMMAD HAJIZADEH, “Characterization and genetic variability of coat protein gene of Apple chlorotic leaf spot virus isolates from apple in Kurdistan and western Azarbaijan Provinces, Iran,” JOURNAL OF CROP PROTECTION, vol. 8, no. 4 , pp. 491–500, 2019, [Online]. Available: https://sid.ir/paper/238923/en

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