Occurrence of Hop stunt viroid in apple orchards of the Northwest Iran

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ROUMI VAHID; Gazel Mona; Caglayan Kadriye

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ISSN: 2588-4824

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Journal: JOURNAL OF CROP PROTECTION Year:2020 | Volume:9 | Issue:2 Page(s): 301-306

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Title

Occurrence of Hop stunt viroid in apple orchards of the Northwest Iran

Author(s)

ROUMI VAHID | Gazel Mona | Caglayan Kadriye | Issue Writer Certificate

Keywords

DetectionQ1

AppleQ1

HSVdQ1

ViroidQ2

IranQ2

Abstract

We investigated the presence of Hop stunt Viroid (HSVd) in Apple samples, showing dappling fruit symptoms, in the Maragheh area (Northwest Iran) by means of RT-PCR. The Viroid was detected only in leaves collected from symptomatic trees and a 298bp amplicon (IR-Gala) was directly sequenced in both directions. Multiple sequence alignment and Blast analyzes revealed that IR-Gala isolate shares the highest identity with grapevine isolates from Brazil and China. Amongst Iranian isolates of HSVd available in the GenBank, this isolate had the highest identity with grapevine isolate of HSVd from Maragheh region. In Phylogenetic analysis by MrBayes, IR-Gala was clustered with grapevine isolates from Brazil, China and Iran and may suggest that HSVd-Apple isolate could be originated from grapevine.

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APA: Copy

ROUMI, VAHID, Gazel, Mona, & Caglayan, Kadriye. (2020). Occurrence of Hop stunt viroid in apple orchards of the Northwest Iran. JOURNAL OF CROP PROTECTION, 9(2 ), 301-306. SID. https://sid.ir/paper/239011/en

Vancouver: Copy

ROUMI VAHID, Gazel Mona, Caglayan Kadriye. Occurrence of Hop stunt viroid in apple orchards of the Northwest Iran. JOURNAL OF CROP PROTECTION[Internet]. 2020;9(2 ):301-306. Available from: https://sid.ir/paper/239011/en

IEEE: Copy

VAHID ROUMI, Mona Gazel, and Kadriye Caglayan, “Occurrence of Hop stunt viroid in apple orchards of the Northwest Iran,” JOURNAL OF CROP PROTECTION, vol. 9, no. 2 , pp. 301–306, 2020, [Online]. Available: https://sid.ir/paper/239011/en

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