GENETIC DIVERSITY OF CYTOSPORA SCHULZERI ISOLATES USING RAPD-PCR AND MP-PCR MARKERS ON APPLES OF SEMIROM REGION OF IRAN

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MEHRABI MEHDI; MOHAMMADI GOLTAPEH EBRAHIM; BERDI FOTOUHIFAR KHALIL

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Journal: JOURNAL OF CROP PROTECTION Year:2015 | Volume:4 | Issue:4 Page(s): 441-452

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Title

GENETIC DIVERSITY OF CYTOSPORA SCHULZERI ISOLATES USING RAPD-PCR AND MP-PCR MARKERS ON APPLES OF SEMIROM REGION OF IRAN

Author(s)

MEHRABI MEHDI | MOHAMMADI GOLTAPEH EBRAHIM | BERDI FOTOUHIFAR KHALIL | Issue Writer Certificate

Keywords

APPLEQ2

CYTOSPORAQ2

GENETIC DIVERSITYQ2

MP-PCRQ1

RAPDQ2

Abstract

GENETIC DIVERSITY relationships of 50 isolates of CYTOSPORA schulzeri on APPLE from different parts of the Semirom region were analyzed using 15 polymerase chain reaction (PCR) based markers, 7 random amplified polymorphic DNAs (RAPDs) and 8 Microsatellite primed polymerase chain reaction (MPPCR).Using 7 selected RAPD primers 113 bands were generated, of which 81 bands were polymorphic (71.7%), with an average of 11.57 polymorphic fragments per primer, and with 8 selected MP-PCR primers 107 amplified bands were observed with 78 polymorphic bands (72.3%), with an average of 9.75 polymorphic fragments per primer. In RAPD marker, number of polymorphic bands varied from 8 (241) to 15 (230, 238, OPA13) with an average of 11.57 per primer and which varied in size from 200 to 3750 bp. Percentage of polymorphism ranged from 64% (203 and 232) to a maximum of 83% (238). In MP-PCR marker, number of polymorphic bands varied from 6 (CAG) to 12 (GTG and ATG) with an average of 9.75 per primer and which varied in size from 200 to 3500 bp.Percentage of polymorphism ranged from 54% (CAG) to a maximum of 81% (ACTG). By combining markers, a total of 220 bands were detected, of which 159 bands (72%) were polymorphic and produced on an average 10.6 polymorphic bands per primer. The results showed that both markers were suitable for the detection of genetic polymorphism among APPLEC. schulzeri isolates. Estimated genetic relationship using similarity co-efficient (Jaccard’s) values between different pair of accessions varied from 0.54 to 0.89 in RAPD, 0.62 to 0.89 in MPPCR and 0.62 to 0.87 with combined markers based similarities. High cophenetic correlation between the similarity matrix and corresponding dendrogram was obtained by RAPD+MP-PCR marker (r=0.81). Cluster analysis of the data using UPGMA based on Jaccard´s similarity coefficient, divided the isolates into six groups, showing a high GENETIC DIVERSITY among populations ofC. schulzeri.

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APA: Copy

MEHRABI, MEHDI, MOHAMMADI GOLTAPEH, EBRAHIM, & BERDI FOTOUHIFAR, KHALIL. (2015). GENETIC DIVERSITY OF CYTOSPORA SCHULZERI ISOLATES USING RAPD-PCR AND MP-PCR MARKERS ON APPLES OF SEMIROM REGION OF IRAN. JOURNAL OF CROP PROTECTION, 4(4), 441-452. SID. https://sid.ir/paper/239034/en

Vancouver: Copy

MEHRABI MEHDI, MOHAMMADI GOLTAPEH EBRAHIM, BERDI FOTOUHIFAR KHALIL. GENETIC DIVERSITY OF CYTOSPORA SCHULZERI ISOLATES USING RAPD-PCR AND MP-PCR MARKERS ON APPLES OF SEMIROM REGION OF IRAN. JOURNAL OF CROP PROTECTION[Internet]. 2015;4(4):441-452. Available from: https://sid.ir/paper/239034/en

IEEE: Copy

MEHDI MEHRABI, EBRAHIM MOHAMMADI GOLTAPEH, and KHALIL BERDI FOTOUHIFAR, “GENETIC DIVERSITY OF CYTOSPORA SCHULZERI ISOLATES USING RAPD-PCR AND MP-PCR MARKERS ON APPLES OF SEMIROM REGION OF IRAN,” JOURNAL OF CROP PROTECTION, vol. 4, no. 4, pp. 441–452, 2015, [Online]. Available: https://sid.ir/paper/239034/en

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