ELIMINATION OF BEAN YELLOW MOSAIC VIRUS THROUGH THERMOTHERAPY COMBINED WITH MERISTEM-TIP CULTURE IN GLADIOLUS CORMS

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SHARIFI NEZAMABAD PARISA; KOOHI HABIBI MINA; DIZADJI AKBAR; KALANTARI SIAMAK

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Journal: JOURNAL OF CROP PROTECTION Year:2015 | Volume:4 | Issue:4 Page(s): 533-543

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Title

ELIMINATION OF BEAN YELLOW MOSAIC VIRUS THROUGH THERMOTHERAPY COMBINED WITH MERISTEM-TIP CULTURE IN GLADIOLUS CORMS

Author(s)

SHARIFI NEZAMABAD PARISA | KOOHI HABIBI MINA | DIZADJI AKBAR | KALANTARI SIAMAK | Issue Writer Certificate

Keywords

BYMVQ2

GLADIOLUSQ2

MERISTEM-TIP CULTUREQ2

THERMOTHERAPYQ2

VIRUS FREE CORMQ2

Abstract

Bean yellow mosaic virus(BYMV, Potyvirus, Potyviridae) causes serious disease inGladiolus spp. In this work, the possibility of obtaining BYMV free plant material from virus infected GLADIOLUS corms was studied. THERMOTHERAPY, meristemtip culture and combination of both techniques on infected corms/meristem-tip explants (0.5–1 mm in length) resulted in BYMV elimination up to 15.38, 78.04 and 86.66%, respectively, as determined by double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) and reverse transcription-polymerase chain reaction (IC-RT-PCR). Individual virus-free shoots readily rooted in vitro and were transferred to corm formation medium. The results showed that THERMOTHERAPY promotes the survival rate of explants during MERISTEM-TIP CULTURE steps (except regeneration step) and also plantlet acclimatization. Statistical analysis showed that the BYMV elimination in GLADIOLUS corms was significantly (P≤ 0.01) affected by THERMOTHERAPY treatment of infected corms. THERMOTHERAPY combined with meristem culture can greatly improve BYMV elimination efficiency from infected GLADIOLUS corms, resulting in the production of BYMV free GLADIOLUS plants.

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APA: Copy

SHARIFI NEZAMABAD, PARISA, KOOHI HABIBI, MINA, DIZADJI, AKBAR, & KALANTARI, SIAMAK. (2015). ELIMINATION OF BEAN YELLOW MOSAIC VIRUS THROUGH THERMOTHERAPY COMBINED WITH MERISTEM-TIP CULTURE IN GLADIOLUS CORMS. JOURNAL OF CROP PROTECTION, 4(4), 533-543. SID. https://sid.ir/paper/239038/en

Vancouver: Copy

SHARIFI NEZAMABAD PARISA, KOOHI HABIBI MINA, DIZADJI AKBAR, KALANTARI SIAMAK. ELIMINATION OF BEAN YELLOW MOSAIC VIRUS THROUGH THERMOTHERAPY COMBINED WITH MERISTEM-TIP CULTURE IN GLADIOLUS CORMS. JOURNAL OF CROP PROTECTION[Internet]. 2015;4(4):533-543. Available from: https://sid.ir/paper/239038/en

IEEE: Copy

PARISA SHARIFI NEZAMABAD, MINA KOOHI HABIBI, AKBAR DIZADJI, and SIAMAK KALANTARI, “ELIMINATION OF BEAN YELLOW MOSAIC VIRUS THROUGH THERMOTHERAPY COMBINED WITH MERISTEM-TIP CULTURE IN GLADIOLUS CORMS,” JOURNAL OF CROP PROTECTION, vol. 4, no. 4, pp. 533–543, 2015, [Online]. Available: https://sid.ir/paper/239038/en

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