FAILURE OF PCR-RAPD TECHNIQUE TO DIFFERENTIATE SEX IN MAHISEFIED (RUTILUS FRISII KUTUM) FROM THE SOUTH CASPIAN SEA

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POURKAZEMI M.; RAZIKAZEMI S.

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Paper Information

Journal: CASPIAN JOURNAL OF ENVIRONMENTAL SCIENCES (CJES) Year:2011 | Volume:9 | Issue:2 Page(s): 235-242

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Title

FAILURE OF PCR-RAPD TECHNIQUE TO DIFFERENTIATE SEX IN MAHISEFIED (RUTILUS FRISII KUTUM) FROM THE SOUTH CASPIAN SEA

Author(s)

POURKAZEMI M. | RAZIKAZEMI S. | Issue Writer Certificate

Keywords

MAHISEFIDQ3

RUTILUS FRISII KUTUMQ3

RANDOM AMPLIFIED POLYMORPHIC DNAQ3

SEX DETERMINATIONQ3

Abstract

In order to identify the sex marker in Mahisefied, RUTILUS FRISII KUTUM, samples from 5 male and 5 female fish were collected from the south Caspian Sea. Polymerase chain reaction RANDOM AMPLIFIED POLYMORPHIC DNA (PCR-RAPD) was performed using 124 primer sets. All bands were numbered using 1 and 0 scores corresponding to the presence or absence of bands, respectively and data were analyzed using RAPDPLOT program. Results indicated that 44 sets of primers did not show any flanking site and produced no bands, while the remaining 80 produced sharp and visible bands on polyacrylamid gel. In total, 1600 bands were scored. However, none of the bands corresponded to either the male or female fish. According to the results it has been concluded that RAPD technique failed to detect sex and cannot be considered as a robust molecular tool for sex differentiation in the studied fish. The reason may be the absence of sex chromosomes in this species or that the genes corresponding to sex differentiation are spread on different autosomal chromosomes with interaction of some environmental factors.

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APA: Copy

POURKAZEMI, M., & RAZIKAZEMI, S.. (2011). FAILURE OF PCR-RAPD TECHNIQUE TO DIFFERENTIATE SEX IN MAHISEFIED (RUTILUS FRISII KUTUM) FROM THE SOUTH CASPIAN SEA. CASPIAN JOURNAL OF ENVIRONMENTAL SCIENCES (CJES), 9(2), 235-242. SID. https://sid.ir/paper/240778/en

Vancouver: Copy

POURKAZEMI M., RAZIKAZEMI S.. FAILURE OF PCR-RAPD TECHNIQUE TO DIFFERENTIATE SEX IN MAHISEFIED (RUTILUS FRISII KUTUM) FROM THE SOUTH CASPIAN SEA. CASPIAN JOURNAL OF ENVIRONMENTAL SCIENCES (CJES)[Internet]. 2011;9(2):235-242. Available from: https://sid.ir/paper/240778/en

IEEE: Copy

M. POURKAZEMI, and S. RAZIKAZEMI, “FAILURE OF PCR-RAPD TECHNIQUE TO DIFFERENTIATE SEX IN MAHISEFIED (RUTILUS FRISII KUTUM) FROM THE SOUTH CASPIAN SEA,” CASPIAN JOURNAL OF ENVIRONMENTAL SCIENCES (CJES), vol. 9, no. 2, pp. 235–242, 2011, [Online]. Available: https://sid.ir/paper/240778/en

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