EVALUATION OF EFFECT OF BACILLUS SUBTILIS AS PROBIOTIC ON HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) FOLLOWING EXPERIMENTAL INFECTION WITH STREPTOCOCCUS INIAE

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KAMGAR M.; GHANE M.; POURGHOLAM R.; GHIASI M.

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Journal: Journal of Aquaculture Development Year:2012 | Volume:6 | Issue:1 Page(s): 91-102

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Title

EVALUATION OF EFFECT OF BACILLUS SUBTILIS AS PROBIOTIC ON HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) FOLLOWING EXPERIMENTAL INFECTION WITH STREPTOCOCCUS INIAE

Author(s)

KAMGAR M. | GHANE M. | POURGHOLAM R. | GHIASI M. | Issue Writer Certificate

Keywords

RAINBOW TROUTQ2

PROBIOTICQ2

STREPTOCOCCOSISQ2

BACILLUS SUBTILISQ2

HEMATOLOGICAL AND BIOCHEMICAL FACTORSQ2

Abstract

In this study BACILLUS SUBTILIS is used for control of STREPTOCOCCOSIS with the agent of Streptococcus iniae in RAINBOW TROUT. The experience was carried out in 2 groups (Control and Treatment) and 3 replicates. In Control group, PROBIOTIC was not applied in diet but in Test group, BACILLUS SUBTILIS was administered in feed at a concentration of 107 cells per gram. In the day of forty five, 0.1 ml intraperitoneally injection of S.iniae with 2×107 cells ml-1 dosage was done for both of groups and were checked for the rest of the survey duration (2 weeks). At the end of the time which took about two months, 5 blood samples were caught for hematological and biochemical experiments from every replicate for realizing the effect of B.subtilis feeding on resistance of fish against S.iniae infection. After injection of S.iniae, there was no significant difference among control and treatment groups considering parameters such as erythrocyte count, hemoglobin, hematocrit, mean cell volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration and percent of monocyte (p>0.05). But significant difference was seen in the leukocyte count, percent of lymphocyte and neutrophile, serum total protein, serum albumin, IgM and lysozyme in both groups (p<0.05). The leukocyte count, percent of lymphocyte, serum total protein, serum albumin, IgM and lysozyme were higher in T group and neutrophile percent was lower in comparison with the control.

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APA: Copy

KAMGAR, M., GHANE, M., POURGHOLAM, R., & GHIASI, M.. (2012). EVALUATION OF EFFECT OF BACILLUS SUBTILIS AS PROBIOTIC ON HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) FOLLOWING EXPERIMENTAL INFECTION WITH STREPTOCOCCUS INIAE. JOURNAL OF AQUACULTURE DEVELOPMENT, 6(1), 91-102. SID. https://sid.ir/paper/242023/en

Vancouver: Copy

KAMGAR M., GHANE M., POURGHOLAM R., GHIASI M.. EVALUATION OF EFFECT OF BACILLUS SUBTILIS AS PROBIOTIC ON HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) FOLLOWING EXPERIMENTAL INFECTION WITH STREPTOCOCCUS INIAE. JOURNAL OF AQUACULTURE DEVELOPMENT[Internet]. 2012;6(1):91-102. Available from: https://sid.ir/paper/242023/en

IEEE: Copy

M. KAMGAR, M. GHANE, R. POURGHOLAM, and M. GHIASI, “EVALUATION OF EFFECT OF BACILLUS SUBTILIS AS PROBIOTIC ON HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) FOLLOWING EXPERIMENTAL INFECTION WITH STREPTOCOCCUS INIAE,” JOURNAL OF AQUACULTURE DEVELOPMENT, vol. 6, no. 1, pp. 91–102, 2012, [Online]. Available: https://sid.ir/paper/242023/en

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