AN EFFECTIVE NUTRIENT MEDIUM FOR ASYMBIOTIC SEED GERMINATION AND IN VITRO SEEDLING DEVELOPMENT OF PHALAENOPSIS ‘BAHIA BLANCA’

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ABBASZADEH SEYEDEH MEHRNAZ; MIRI SEIED MEHDI; NADERI ROUHANGIZ

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Journal: JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS) Year:2018 | Volume:8 | Issue:3 Page(s): 183-192

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Information Journal Paper

Title

AN EFFECTIVE NUTRIENT MEDIUM FOR ASYMBIOTIC SEED GERMINATION AND IN VITRO SEEDLING DEVELOPMENT OF PHALAENOPSIS ‘BAHIA BLANCA’

Author(s)

ABBASZADEH SEYEDEH MEHRNAZ | MIRI SEIED MEHDI | NADERI ROUHANGIZ | Issue Writer Certificate

Keywords

COCONUT WATERQ1

MEDIAQ2

ORCHIDQ1

PEPTONEQ2

PROTOCORM-LIKE BODIESQ1

Abstract

The objective of the present study was to develop a rapid and successful method for promoting asymbiotic seed germination and growth and development of PROTOCORM-LIKE BODIES (PLBs) and subsequent plantlets of Phalaenopsis ORCHID. Four basal MEDIA, i.e. Murashige and Skoog (MS), half-strength MS (.MS), Knudson C (KC), and Vacin and Went (VW) supplemented with COCONUT WATER and PEPTONE, were examined for their effectiveness in large-scale multiplication by asymbiotic seed germination.Seed germination, leaf number, root number, root length, seedling length, and fresh and dry weight were scored. Well-rooted plantlets 4-cm in height were transferred to a greenhouse in trays containing expanded clay, charcoal, cocopeat and perlite (4: 2: 1: 1) or sphagnum moss. The seed germination frequency and seedling development were found to be significantly higher in organic growth supplement-enriched MS and.MS MEDIA. The results indicated that the highest seed germination (94%) was obtained in MS medium supplemented with 100 mg/L COCONUT WATER or 1 g/L PEPTONE and .MS containing 1 or 2 g/L PEPTONE. However, the longest seedling was found in MS or.MS MEDIA supplemented with 100 mg/L COCONUT WATER or MS containing 1 g/L PEPTONE. Ninety-seven percent of plantlets survived after 1 month in mixture MEDIA expanded clay: charcoal: cocopeat: perlite.

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APA: Copy

ABBASZADEH, SEYEDEH MEHRNAZ, MIRI, SEIED MEHDI, & NADERI, ROUHANGIZ. (2018). AN EFFECTIVE NUTRIENT MEDIUM FOR ASYMBIOTIC SEED GERMINATION AND IN VITRO SEEDLING DEVELOPMENT OF PHALAENOPSIS ‘BAHIA BLANCA’. JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS), 8(3 ), 183-192. SID. https://sid.ir/paper/242580/en

Vancouver: Copy

ABBASZADEH SEYEDEH MEHRNAZ, MIRI SEIED MEHDI, NADERI ROUHANGIZ. AN EFFECTIVE NUTRIENT MEDIUM FOR ASYMBIOTIC SEED GERMINATION AND IN VITRO SEEDLING DEVELOPMENT OF PHALAENOPSIS ‘BAHIA BLANCA’. JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS)[Internet]. 2018;8(3 ):183-192. Available from: https://sid.ir/paper/242580/en

IEEE: Copy

SEYEDEH MEHRNAZ ABBASZADEH, SEIED MEHDI MIRI, and ROUHANGIZ NADERI, “AN EFFECTIVE NUTRIENT MEDIUM FOR ASYMBIOTIC SEED GERMINATION AND IN VITRO SEEDLING DEVELOPMENT OF PHALAENOPSIS ‘BAHIA BLANCA’,” JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS), vol. 8, no. 3 , pp. 183–192, 2018, [Online]. Available: https://sid.ir/paper/242580/en

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