Effect of different thawing procedures on frozen semen quality of Ghezel rams

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VATANKHAH S.; DAGHIGH KIA H.; MOGHADDAM G.; EBRAHIMI M.

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Journal: JOURNAL OF RUMINANT RESEARCH Year:2018 | Volume:6 | Issue:2 Page(s): 117-132

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Title

Effect of different thawing procedures on frozen semen quality of Ghezel rams

Author(s)

VATANKHAH S. | DAGHIGH KIA H. | MOGHADDAM G. | EBRAHIMI M. | Issue Writer Certificate

Keywords

Thawing ratesQ1

Quality parametersQ2

Frozen semenQ2

RamQ1

Abstract

Background and objectives: Today, artificial insemination has a special place in improving production efficiency and improving genetic progress. In order to realize many of the potential benefits of artificial insemination, semen storage for a long-term is considered a necessary step by freezing process. The freezing process, including the thawing process, leads to morphological changes, damage to the natural functions of the sperm and ultimately reduced fertility. Therefore, improving the thawing method of Ram semen is related to the sperm quality indicators and is of considerable importance. The purpose of this study was to compare the effects of two thawing procedures on microscopic paRameters, activity of Glutathione peroxidase and Superoxidase dismutase enzymes, and total antioxidant capacity of Ram semen after freeze-thawing process. Materials and methods: In this study, semen samples were collected from five Ghezel Ram (3 and 4 years of age), during the breeding season, using an artificial vagina twice a week. To eliminate the individual effects of animals, semen samples were pooled after each sampling. Then diluted with Tris diluents having 1. 5% soyben lecetin and 7% glycerol. The diluted semen’ s were filled at 0. 25 ml straw and frozen in nitrogen vapor and stored in a tank containing liquid nitrogen until evaluation. The straws were thawed at water bath temperatures at (i) 37º C for 30 s and (ii) 60º C for 6 s. Following the freeze-thawing process, the effects of time exposure and thawing temperature on the kinematic features (CASA), viability, plasma membrane integrity, morphological abnormalities, malondialdehyde concentration, antioxidant activity, and total antioxidant capacity were evaluated. The experiment was conducted as a completely randomized and the statistical model includes the effect of temperature and thawing time. The data were analyzed by the general linear model (GLM) procedure and statistical differences between the various groups means were determined by Tukey's test. Significant differences were reported at the level of (P<0. 05). Results: The results showed that sperm motility paRameters such as progressive motility, straight-line velocity, and curvilinear velocity at 37° C to 60 ° c were not statistically significant difference, but, there were some changes in terms of numbers. The viability and plasma membrane integrity at 37° C for 30 s compared 60° C for 6 s were positively changed numerically, but it was no significant. Also, total abnormality did not improve at high Thawing rates than low rates. In addition, the results of the comparison of lipid peroxidation, glutathione peroxidase, superoxide dismutase and total antioxidant capacity were no significant at two thawing procedures. Conclusion: According to the results obtained from thawing methods, the motility index, viability, and oxidative status at thawing rate 60° C for 6 s cannot be a suitable alternative for semen thawing at 37° C for 30 seconds.

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APA: Copy

VATANKHAH, S., DAGHIGH KIA, H., MOGHADDAM, G., & EBRAHIMI, M.. (2018). Effect of different thawing procedures on frozen semen quality of Ghezel rams. JOURNAL OF RUMINANT RESEARCH, 6(2 ), 117-132. SID. https://sid.ir/paper/244014/en

Vancouver: Copy

VATANKHAH S., DAGHIGH KIA H., MOGHADDAM G., EBRAHIMI M.. Effect of different thawing procedures on frozen semen quality of Ghezel rams. JOURNAL OF RUMINANT RESEARCH[Internet]. 2018;6(2 ):117-132. Available from: https://sid.ir/paper/244014/en

IEEE: Copy

S. VATANKHAH, H. DAGHIGH KIA, G. MOGHADDAM, and M. EBRAHIMI, “Effect of different thawing procedures on frozen semen quality of Ghezel rams,” JOURNAL OF RUMINANT RESEARCH, vol. 6, no. 2 , pp. 117–132, 2018, [Online]. Available: https://sid.ir/paper/244014/en

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