STUDY ON ASSOCIATION OF LEPTIN AND GROWTH HORMONE RECEPTOR GENES POLYMORPHISMS WITH SEMEN QUALITY OF HOLSTEIN BULLS

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ASGARI M.; ALIJANI S.; JAVANMARD A.; DAGHIGH KIA H.

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Journal: JOURNAL OF RUMINANT RESEARCH Year:2015 | Volume:3 | Issue:3 Page(s): 169-184

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Title

STUDY ON ASSOCIATION OF LEPTIN AND GROWTH HORMONE RECEPTOR GENES POLYMORPHISMS WITH SEMEN QUALITY OF HOLSTEIN BULLS

Author(s)

ASGARI M. | ALIJANI S. | JAVANMARD A. | DAGHIGH KIA H. | Issue Writer Certificate

Keywords

SEMENQ2

HOLSTEINSQ2

LEPTINQ2

GROWTH HORMONE RECEPTORQ2

POLYMORPHISMQ2

Abstract

Object and Background: The purpose of this study was to evaluate the POLYMORPHISM of two candidate genes including LEPTIN (exon 2 and intron 2) and GROWTH HORMONE RECEPTOR (promoter) genes and their effects on SEMEN quality of Holstein bulls.Materials and Methods: For this purpose information from two breeding stations including the Animal Breeding Center of Northwest (41bulls) and the National Animal Breeding Center and Promotion of Animal Products (28 bulls) were used. Information of 69 bulls, which were sampled between 2003-2013 years was used. Four qualitative sperm traits including volume of ejaculation (mL), sperm population (million/mL), and percentages of live sperm before and after freezing (%) were measured. POLYMORPHISM of LEPTIN gene was evaluated by PCR-RFLP and the variation in the GROWTH HORMONE RECEPTOR gene was evaluated by PCR-SSR.Results: The results of this study showed that all investigated loci were polymorphic and the frequency of TT, AT, AA genotypes in exon 2 were 0.28, 0.20 and 0.52, and frequency of T and A alleles, were 0.62±0.041 and 0.38±0.041, respectively. Additionally, genotype frequencies for AA, AB, and BB genotypes in the intron 2 were estimated 0.68, 0.32 and 0.16 and estimated A and B allele frequency were 0.84±0.031 and 0.16±0.031 respectively. For promoter of GROWTH HORMONE RECEPTOR, results indicated that three genotypes of TG17/TG17, TG17/TG21 and TG21/TG21 had frequencies of 0.27, 0.48 and 0.25 and two alleles of TG21 and TG17 had frequencies of 0.49±0.042 and 0.51±0.042, respectively. There was Hardy-Weinberg equilibrium for the promoter of GROWTH HORMONE RECEPTOR (P<0.67) and intron 2 of LEPTIN (P<0.12), while there was no Hardy-Weinberg equilibrium for the variation of exon 2 of LEPTIN (P<0.01).Conclusion: Generally, there was a significant association (P<0.01) between POLYMORPHISMs in all loci and SEMEN quality traits. Specially, in exon 2 of LEPTIN, the AA genotype was the favorable genotype for SEMEN volume and percentage of live sperm before freezing and the TT genotype was the favorable genotype for sperm population and percentage of live sperm after freezing. For intron 2 of LEPTIN, the AA genotype was the best genotype for all traits. In promoter of GROWTH HORMONE RECEPTOR, the genotype of TG17/TG17 was the favorable for all traits except for SEMEN volume and TG21/TG21 was the best genotype for SEMEN volume. Also the result of this study can be used in marker-assisted selection to improve expressed traits.

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APA: Copy

ASGARI, M., ALIJANI, S., JAVANMARD, A., & DAGHIGH KIA, H.. (2015). STUDY ON ASSOCIATION OF LEPTIN AND GROWTH HORMONE RECEPTOR GENES POLYMORPHISMS WITH SEMEN QUALITY OF HOLSTEIN BULLS. JOURNAL OF RUMINANT RESEARCH, 3(3), 169-184. SID. https://sid.ir/paper/244108/en

Vancouver: Copy

ASGARI M., ALIJANI S., JAVANMARD A., DAGHIGH KIA H.. STUDY ON ASSOCIATION OF LEPTIN AND GROWTH HORMONE RECEPTOR GENES POLYMORPHISMS WITH SEMEN QUALITY OF HOLSTEIN BULLS. JOURNAL OF RUMINANT RESEARCH[Internet]. 2015;3(3):169-184. Available from: https://sid.ir/paper/244108/en

IEEE: Copy

M. ASGARI, S. ALIJANI, A. JAVANMARD, and H. DAGHIGH KIA, “STUDY ON ASSOCIATION OF LEPTIN AND GROWTH HORMONE RECEPTOR GENES POLYMORPHISMS WITH SEMEN QUALITY OF HOLSTEIN BULLS,” JOURNAL OF RUMINANT RESEARCH, vol. 3, no. 3, pp. 169–184, 2015, [Online]. Available: https://sid.ir/paper/244108/en

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