INVESTIGATING THE GENETIC DIVERSITY IN SOME OF CULTIVATED AND WILD BARELY GENOTYPES USING AFLP MOLECULAR MARKERS

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HOSSEINI MASOUMEH; GHORBANLI MAHLAGHA; SABOURI HOSSEIN; DADRAS AHMAD REZA; SATTARIAN ALI; FALLAHI HOSSEIN ALI

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Journal: Cereal Research Year:2017 | Volume:6 | Issue:4 Page(s): 533-544

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Title

INVESTIGATING THE GENETIC DIVERSITY IN SOME OF CULTIVATED AND WILD BARELY GENOTYPES USING AFLP MOLECULAR MARKERS

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CLUSTER ANALYSISQ2

NEI’S GENE DIVERSITYQ1

POLYMORPHIC INFORMATION CONTENTQ2

SHANON’S INDEXQ1

Abstract

In this study, AFLP markers were used to investigate the genetic diversity among 24 genotypes of barley. The eight primer combinations of Eco RI/ Tru1I, totally produced 332 scorable bands that 292 (89.15 %) were polymorph. Genetic diversity was estimated between 0.29-0.38 using Nei's gene diversity coefficient. Also, genetic similarity of the genotypes varied from 0.20.0 to 0.56. The highest (0.47 %) and lowest (0.26 %) polymorphism were obtained using primer combinations of Eco RI+ACG Tru1I+ CGA and Eco RI+ACT- Tru1I+CCG, respectively. CLUSTER ANALYSIS using Jaccard coefficient and UPGMA method assigned genotypes to four main groups. In the first and second groups there were sixrows genotypes and in the third and fourth groups a combination of six- and two-rows genotypes. On the based on similarity matrix the genetic distance between Nimrouz and genotype population Khorasan- Razavi also genotype population of Karaje TN2173 and Line EB-86-3 was low, while the genetic distance between genotype population of Tilabad and cross (F1) ALISOS/CI03909-2 was high. Considering to achieving high rates of polymorphism in the present study can use AFLP marker specially primer combination such as ACG-Tru1I+CGA+Eco RI which produced high level of polymorphism as a powerful tool to distinguish of close genotypes and other barley breeding programs.

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APA: Copy

HOSSEINI, MASOUMEH, GHORBANLI, MAHLAGHA, SABOURI, HOSSEIN, DADRAS, AHMAD REZA, SATTARIAN, ALI, & FALLAHI, HOSSEIN ALI. (2017). INVESTIGATING THE GENETIC DIVERSITY IN SOME OF CULTIVATED AND WILD BARELY GENOTYPES USING AFLP MOLECULAR MARKERS. CEREAL RESEARCH, 6(4), 533-544. SID. https://sid.ir/paper/246682/en

Vancouver: Copy

HOSSEINI MASOUMEH, GHORBANLI MAHLAGHA, SABOURI HOSSEIN, DADRAS AHMAD REZA, SATTARIAN ALI, FALLAHI HOSSEIN ALI. INVESTIGATING THE GENETIC DIVERSITY IN SOME OF CULTIVATED AND WILD BARELY GENOTYPES USING AFLP MOLECULAR MARKERS. CEREAL RESEARCH[Internet]. 2017;6(4):533-544. Available from: https://sid.ir/paper/246682/en

IEEE: Copy

MASOUMEH HOSSEINI, MAHLAGHA GHORBANLI, HOSSEIN SABOURI, AHMAD REZA DADRAS, ALI SATTARIAN, and HOSSEIN ALI FALLAHI, “INVESTIGATING THE GENETIC DIVERSITY IN SOME OF CULTIVATED AND WILD BARELY GENOTYPES USING AFLP MOLECULAR MARKERS,” CEREAL RESEARCH, vol. 6, no. 4, pp. 533–544, 2017, [Online]. Available: https://sid.ir/paper/246682/en

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