ELECTROPHORESIS PATTERN OF TOTAL PROTEIN, OUTER MEMBRANE PROTEIN AND LIPOPOLYSACCHARIDE OF YERSINIA RUCKERI ISOLATES IN SOME FARMED RAINBOW TROUT (ONCHORHYNCHUS MYKISS) IN IRAN

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SOLTANI MEHDI; MOUSAVI SHALALEH; SHAFIEI SHAFIGH; TAHERI MOHAMMAD

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Paper Information

Journal: JOURNAL OF FISHERIES SCIENCE AND TECHNOLOGY (JFST) Year:2016 | Volume:5 | Issue:1 Page(s): 131-140

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Title

ELECTROPHORESIS PATTERN OF TOTAL PROTEIN, OUTER MEMBRANE PROTEIN AND LIPOPOLYSACCHARIDE OF YERSINIA RUCKERI ISOLATES IN SOME FARMED RAINBOW TROUT (ONCHORHYNCHUS MYKISS) IN IRAN

Author(s)

SOLTANI MEHDI | MOUSAVI SHALALEH | SHAFIEI SHAFIGH | TAHERI MOHAMMAD | Issue Writer Certificate

Keywords

YERSINIA RUCKERIQ2

RAINBOW TROUTQ2

PROTEIN PROFILEQ1

OUTER MEMBRANEQ2

LIPOPOLYSACCHARIDEQ2

Abstract

The molecular characteristics of YERSINIA RUCKERI such as total proteins (TP), OUTER MEMBRANE proteins OMP) and LIPOPOLYSACCHARIDEs (LPS) in 34 isolates from RAINBOW TROUT farms in Tehran, Mazandaran and Zanjan provinces were determined, using SDS-PAGE method. The molecular weight (MW) for TP of all bacterial isolates was mostly less than 100 KD with a banding density in range 28 to 100 KD. Also, protein pattern of OMP consisted of three major bands with MW of 28-35 KD (two bands) and 10-17 KD (one band) plus some minor bands with MW of 48-75 KD and 17-28 KD. In addition, the LPS pattern of all bacterial isolates were less than 130 KD with the most band density in range 28-100 KD. These results show that the banding profile of TP, OMP and LPS of all isolates of Y. ruckeri were identical, demonstrating minimum heterogeneity among Iranian isolates of Y. ruckeri. Therefore, it is feasible for the formulation of a monovalent vaccine to yersiniosis in future.

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APA: Copy

SOLTANI, MEHDI, MOUSAVI, SHALALEH, SHAFIEI, SHAFIGH, & TAHERI, MOHAMMAD. (2016). ELECTROPHORESIS PATTERN OF TOTAL PROTEIN, OUTER MEMBRANE PROTEIN AND LIPOPOLYSACCHARIDE OF YERSINIA RUCKERI ISOLATES IN SOME FARMED RAINBOW TROUT (ONCHORHYNCHUS MYKISS) IN IRAN. JOURNAL OF FISHERIES SCIENCE AND TECHNOLOGY (JFST), 5(1), 131-140. SID. https://sid.ir/paper/246881/en

Vancouver: Copy

SOLTANI MEHDI, MOUSAVI SHALALEH, SHAFIEI SHAFIGH, TAHERI MOHAMMAD. ELECTROPHORESIS PATTERN OF TOTAL PROTEIN, OUTER MEMBRANE PROTEIN AND LIPOPOLYSACCHARIDE OF YERSINIA RUCKERI ISOLATES IN SOME FARMED RAINBOW TROUT (ONCHORHYNCHUS MYKISS) IN IRAN. JOURNAL OF FISHERIES SCIENCE AND TECHNOLOGY (JFST)[Internet]. 2016;5(1):131-140. Available from: https://sid.ir/paper/246881/en

IEEE: Copy

MEHDI SOLTANI, SHALALEH MOUSAVI, SHAFIGH SHAFIEI, and MOHAMMAD TAHERI, “ELECTROPHORESIS PATTERN OF TOTAL PROTEIN, OUTER MEMBRANE PROTEIN AND LIPOPOLYSACCHARIDE OF YERSINIA RUCKERI ISOLATES IN SOME FARMED RAINBOW TROUT (ONCHORHYNCHUS MYKISS) IN IRAN,” JOURNAL OF FISHERIES SCIENCE AND TECHNOLOGY (JFST), vol. 5, no. 1, pp. 131–140, 2016, [Online]. Available: https://sid.ir/paper/246881/en

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