SHORT COMMUNICATION: ANALYSIS OF GROWTH HORMONE GENE IN ALPINE AND SAANEN GOATS USING PCR-SSCP METHOD

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KHATAMI NEJHAD R.; YOUSEFI S.; AHANI AZARI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE Year:2014 | Volume:4 | Issue:1 Page(s): 201-203

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Title

SHORT COMMUNICATION: ANALYSIS OF GROWTH HORMONE GENE IN ALPINE AND SAANEN GOATS USING PCR-SSCP METHOD

Author(s)

KHATAMI NEJHAD R. | YOUSEFI S. | AHANI AZARI M. | Issue Writer Certificate

Keywords

CAPRINE GH GENEQ2

GENETIC POLYMORPHISMQ2

GOATQ2

Abstract

In this study the GENETIC POLYMORPHISM of growth hormone (GH) gene as a candidate gene in livestock was investigated. Blood samples were randomly collected from 34 Alpine and 42 Saanen GOATs. DNA was extracted from blood samples and a 365 bp region of exon 5 of the GH gene was amplified by polymerase chain reaction (PCR). PCR products were analyzed using single strand conformation polymorphism (SSCP) method on polyacrylamide gel. The results indicated that there are four (G1, G2, G3 and G4) conformational patterns with frequencies 0.38, 0.21, 0.23 and 0.18 for Alpine GOAT and 0.48, 0.21, 0.17 and 0.14 for Saanen GOAT, respectively. These results revealed that GH gene was polymorph in this study and showed that PCR-SSCP is an appropriate tool for detecting poly-morphism and evaluating genetic variability.

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APA: Copy

KHATAMI NEJHAD, R., YOUSEFI, S., & AHANI AZARI, M.. (2014). SHORT COMMUNICATION: ANALYSIS OF GROWTH HORMONE GENE IN ALPINE AND SAANEN GOATS USING PCR-SSCP METHOD. IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE, 4(1), 201-203. SID. https://sid.ir/paper/249806/en

Vancouver: Copy

KHATAMI NEJHAD R., YOUSEFI S., AHANI AZARI M.. SHORT COMMUNICATION: ANALYSIS OF GROWTH HORMONE GENE IN ALPINE AND SAANEN GOATS USING PCR-SSCP METHOD. IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE[Internet]. 2014;4(1):201-203. Available from: https://sid.ir/paper/249806/en

IEEE: Copy

R. KHATAMI NEJHAD, S. YOUSEFI, and M. AHANI AZARI, “SHORT COMMUNICATION: ANALYSIS OF GROWTH HORMONE GENE IN ALPINE AND SAANEN GOATS USING PCR-SSCP METHOD,” IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE, vol. 4, no. 1, pp. 201–203, 2014, [Online]. Available: https://sid.ir/paper/249806/en

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