Enhancement of valerenic acid production in Valeriana officinalis roots by methyl jasmonate-mediated transcriptional changes of sesquiterpene synthase genes

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TAHERI HENGAMEH; GHESMATI MANSOUR

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Journal: IRANIAN JOURNAL OF PLANT PHYSIOLOGY Year:2019 | Volume:9 | Issue:1 Page(s): 2611-2618

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Title

Enhancement of valerenic acid production in Valeriana officinalis roots by methyl jasmonate-mediated transcriptional changes of sesquiterpene synthase genes

Author(s)

TAHERI HENGAMEH | GHESMATI MANSOUR | Issue Writer Certificate

Keywords

Valeriana officinalisQ1

sesquiterpenes synthaseQ1

expression patternQ1

quantitative real time PCRQ1

Abstract

Valeriana officinalis (valerian), as a nutraceutical herb, is widely used for its sedative and hypnotic properties. It is known that C15 sesquiterpenoid valerenic acid (VA) is the active ingredient responsible for pharmacological effects of V. officinalis. To evaluate the effect of methyl jasmonate (MeJA) concentrations (50 and 100 μ M) in the modulation of expression patterns of the genes involved in valerenic acid (VA) biosynthesis, transcript abundance of identified sesquiterpene synthase (Sesqui-TPS) genes in the root of V. officinalis was monitored by quantitative real time PCR (qRT-PCR) within a 144 h time period. In addition, Valerenic acid contents were measured by high-performance liquid chromatography (HPLC). The highest amount of VA (12. 45 mg/g dry weight (DW)) was found at 100 μ M MeJA with a 12 fold increase over control culture (1. 03 mg/g DW) at exposure time of 72 h. Moreover, MeJA in a concentration dependent manner enhanced transcription rate of VoTPS1 and VoTPS7 genes. Accordingly, exposure to 100 μ M MeJA for 24 h can be more effective in the induction of these genes than that observed for 50 μ M. Such enhancement was correlated with increased VA accumulation suggesting that these genes may be responsible for the biosynthesis of intermediates involved in the VA-biosynthetic pathway. However, MeJA treatment seemed to have a less significant effect on VoTPS3 expression than VoTPS1 and VoTPS7 genes. This results provide insights for more effective biosynthesis of VA by MeJA-mediated transcriptional changes of putative sesqui-TPS.

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APA: Copy

TAHERI, HENGAMEH, & GHESMATI, MANSOUR. (2019). Enhancement of valerenic acid production in Valeriana officinalis roots by methyl jasmonate-mediated transcriptional changes of sesquiterpene synthase genes. IRANIAN JOURNAL OF PLANT PHYSIOLOGY, 9(1 ), 2611-2618. SID. https://sid.ir/paper/259016/en

Vancouver: Copy

TAHERI HENGAMEH, GHESMATI MANSOUR. Enhancement of valerenic acid production in Valeriana officinalis roots by methyl jasmonate-mediated transcriptional changes of sesquiterpene synthase genes. IRANIAN JOURNAL OF PLANT PHYSIOLOGY[Internet]. 2019;9(1 ):2611-2618. Available from: https://sid.ir/paper/259016/en

IEEE: Copy

HENGAMEH TAHERI, and MANSOUR GHESMATI, “Enhancement of valerenic acid production in Valeriana officinalis roots by methyl jasmonate-mediated transcriptional changes of sesquiterpene synthase genes,” IRANIAN JOURNAL OF PLANT PHYSIOLOGY, vol. 9, no. 1 , pp. 2611–2618, 2019, [Online]. Available: https://sid.ir/paper/259016/en

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