PROMOTION OF CALLUS INITIATION, SHOOT REGENERATION AND PROLIFERATION IN LISIANTHUS

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MIRI SEIED MEHDI; SAVARI AKRAM; BEHZAD KAMELIA; MOHAJER IRAVANI BEHRAD

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Journal: IRANIAN JOURNAL OF PLANT PHYSIOLOGY Year:2016 | Volume:6 | Issue:4 Page(s): 1855-1860

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Information Journal Paper

Title

PROMOTION OF CALLUS INITIATION, SHOOT REGENERATION AND PROLIFERATION IN LISIANTHUS

Author(s)

MIRI SEIED MEHDI | SAVARI AKRAM | BEHZAD KAMELIA | MOHAJER IRAVANI BEHRAD | Issue Writer Certificate

Keywords

LISIANTHUSQ1

CALLUS INITIATIONQ1

SHOOT REGENERATIONQ1

PROLIFERATIONQ2

BAQ2

NAAQ2

Abstract

The effects of plant growth regulators were examined in order to optimize the callus induction, regeneration, and PROLIFERATION of LISIANTHUS (Eustoma grandiflorum). In vitro leaves provided the explants for callus induction. Explants were cultured on Murashige and Skoog (MS) medium with different concentrations of indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D). Maximum callogenesis was obtained on MS medium supplemented with 100 mM NAA. Calluses were cultured on MS medium containing 6-benzyladenin (BA) (4.4, 13.3 or 22.2 mM) with or without 0.5 mM IAA and NAA for regeneration. The highest number of shoots (12.3 shoots/explant) developed on MS media with 22.2 mM BA plus 0.5 mM NAA. Individual shoots 1 cm in length were excised and multiplied. The maximal shoot PROLIFERATION with an average of 10.2 and 11.2 shoots/explant after 4 weeks of culture was achieved when the shoot tips were cultured on MS medium supplemented with 2.2 mM BA with or without 0.5 mM NAA. These results indicate that an efficient callus induction and micropropagation protocol of LISIANTHUS had been established.

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APA: Copy

MIRI, SEIED MEHDI, SAVARI, AKRAM, BEHZAD, KAMELIA, & MOHAJER IRAVANI, BEHRAD. (2016). PROMOTION OF CALLUS INITIATION, SHOOT REGENERATION AND PROLIFERATION IN LISIANTHUS. IRANIAN JOURNAL OF PLANT PHYSIOLOGY, 6(4), 1855-1860. SID. https://sid.ir/paper/259046/en

Vancouver: Copy

MIRI SEIED MEHDI, SAVARI AKRAM, BEHZAD KAMELIA, MOHAJER IRAVANI BEHRAD. PROMOTION OF CALLUS INITIATION, SHOOT REGENERATION AND PROLIFERATION IN LISIANTHUS. IRANIAN JOURNAL OF PLANT PHYSIOLOGY[Internet]. 2016;6(4):1855-1860. Available from: https://sid.ir/paper/259046/en

IEEE: Copy

SEIED MEHDI MIRI, AKRAM SAVARI, KAMELIA BEHZAD, and BEHRAD MOHAJER IRAVANI, “PROMOTION OF CALLUS INITIATION, SHOOT REGENERATION AND PROLIFERATION IN LISIANTHUS,” IRANIAN JOURNAL OF PLANT PHYSIOLOGY, vol. 6, no. 4, pp. 1855–1860, 2016, [Online]. Available: https://sid.ir/paper/259046/en

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