OPTIMIZATION OF A WETTABLE POWDER FORMULATION FOR TWO NATIVE BACILLUS THURINGIENSIS STRAINS

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SALEHI JOUZANI GHOLAMREZA; MOAVEN ELHAM; MORSALI HASAN

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Journal: BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES Year:2014 | Volume:3 | Issue:1 Page(s): 7-15

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Title

OPTIMIZATION OF A WETTABLE POWDER FORMULATION FOR TWO NATIVE BACILLUS THURINGIENSIS STRAINS

Author(s)

SALEHI JOUZANI GHOLAMREZA | MOAVEN ELHAM | MORSALI HASAN | Issue Writer Certificate

Keywords

BACILLUS THURINGIENSISQ2

BIOPESTICIDEQ2

FORMULATIONQ2

WETTABLE POWDERQ2

Abstract

The objective of the present study was to optimize a WETTABLE POWDER FORMULATION for two Iranian Bt strains (YD5 and KH4) in order to enhance efficiency and durability of the final products as biological agent against lepidopteran and coleopteran pests. Massproduction of the mentioned strains was done in a batch fermenter based on the previouslyoptimized economical medium and fermentation conditions. The final concentration was achieved 6 ×109 (CFU/mg). To optimize FORMULATION for two strains, 25% spore crystal mixture of the strain and 75% adjuvants including different fillers, surfactants, materialsenhancing durability, anti UV materials, suspensors, wetters, palatability materials and antimicrobial materials in the framework of five statistical treatments were used. Based on the results, the treatment containing milk powder (4%), perlite (6%), irosile (2%), sodiumloril sulfate (8%), alkaline naphthalene (20%), starch (10%), gelatin (9%), sugar (10%), potassium surbate (1%), casein and gluten (2%), potassium dioxide (3%) and monosodiumglutamate (0.2%) showed maximum suspensibility (73 and 71%) and wettability (25 and24%) for YD5 and KH4 strains, respectively, and selected for the next experiments. Theselected FORMULATION for both strains had a white to grey color, 6-7% moisture content, andpH 6.1 and 6.2. Crystals in both FORMULATIONs were stable without any changes during oneyear. The LC50 of the selected FORMULATION for YD5 and KH4 strains against larvae cottonbollworm and elm leaf beetle was 550 and 510 ng/cm2 leaf, respectively.

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APA: Copy

SALEHI JOUZANI, GHOLAMREZA, MOAVEN, ELHAM, & MORSALI, HASAN. (2014). OPTIMIZATION OF A WETTABLE POWDER FORMULATION FOR TWO NATIVE BACILLUS THURINGIENSIS STRAINS. BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES, 3(1), 7-15. SID. https://sid.ir/paper/261439/en

Vancouver: Copy

SALEHI JOUZANI GHOLAMREZA, MOAVEN ELHAM, MORSALI HASAN. OPTIMIZATION OF A WETTABLE POWDER FORMULATION FOR TWO NATIVE BACILLUS THURINGIENSIS STRAINS. BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES[Internet]. 2014;3(1):7-15. Available from: https://sid.ir/paper/261439/en

IEEE: Copy

GHOLAMREZA SALEHI JOUZANI, ELHAM MOAVEN, and HASAN MORSALI, “OPTIMIZATION OF A WETTABLE POWDER FORMULATION FOR TWO NATIVE BACILLUS THURINGIENSIS STRAINS,” BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES, vol. 3, no. 1, pp. 7–15, 2014, [Online]. Available: https://sid.ir/paper/261439/en

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