Effects of new synthetic cryoprotectant agents on histological characteristics of various classes of vitrified bovine pre-antral follicles

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Shahsavari Mohammad Hamed; MOGHADDAM GHOLAMALI; DAGHIGH KIA HOSSEIN; Ribeiro Rodrigues Ana Paula

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Journal: VETERINARY RESEARCH FORUM Year:2019 | Volume:10 | Issue:1 Page(s): 9-16

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Title

Effects of new synthetic cryoprotectant agents on histological characteristics of various classes of vitrified bovine pre-antral follicles

Author(s)

Shahsavari Mohammad Hamed | MOGHADDAM GHOLAMALI | DAGHIGH KIA HOSSEIN | Ribeiro Rodrigues Ana Paula | Issue Writer Certificate

Keywords

CryopreservationQ2

Freezing mediaQ1

MorphologyQ2

Pre-antral follicleQ1

Abstract

Previous studies have reported many discrepancies about the best type and concentration of cryoprotective agents (CPAs) and biological variability among various Pre-antral follicle classes after Cryopreservation of ovarian tissue. The aim of this study was to investigate the impacts of some synthetic polymers on histological characteristics of different types of Pre-antral follicles after bovine ovarian tissue vitrification. From each bovine ovarian pair, fragments were recovered and immediately fixed for analysis (fresh control group) or submitted to vitrification (sucrose, X-1000, Z-1000 and polyvinylpyrrolidone groups), either followed by in vitro culture for 1 or 5 days. In this case, although, the addition of X-1000 resulted in greater percentages of normal follicles for almost all Pre-antral follicle classes compared to those of other groups, there are some exceptions. These results indicate that the inclusion of polyvinylpyrrolidone in the Freezing media can improve the Morphology of the post-warmed transitional follicles and cultured primordial follicles on day five more than other CPAs. According to the results of this study, it can be concluded that although ovarian tissue Cryopreservation is often performed to preserve the primordial follicles, by choosing the best combination of permeating and non-permeating CPAs (synthetic polymers), more advanced stages of bovine Pre-antral follicles, transitional, primary and secondary follicles, may also survive the Cryopreservation process.

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APA: Copy

Shahsavari, Mohammad Hamed, MOGHADDAM, GHOLAMALI, DAGHIGH KIA, HOSSEIN, & Ribeiro Rodrigues, Ana Paula. (2019). Effects of new synthetic cryoprotectant agents on histological characteristics of various classes of vitrified bovine pre-antral follicles. VETERINARY RESEARCH FORUM, 10(1 ), 9-16. SID. https://sid.ir/paper/263621/en

Vancouver: Copy

Shahsavari Mohammad Hamed, MOGHADDAM GHOLAMALI, DAGHIGH KIA HOSSEIN, Ribeiro Rodrigues Ana Paula. Effects of new synthetic cryoprotectant agents on histological characteristics of various classes of vitrified bovine pre-antral follicles. VETERINARY RESEARCH FORUM[Internet]. 2019;10(1 ):9-16. Available from: https://sid.ir/paper/263621/en

IEEE: Copy

Mohammad Hamed Shahsavari, GHOLAMALI MOGHADDAM, HOSSEIN DAGHIGH KIA, and Ana Paula Ribeiro Rodrigues, “Effects of new synthetic cryoprotectant agents on histological characteristics of various classes of vitrified bovine pre-antral follicles,” VETERINARY RESEARCH FORUM, vol. 10, no. 1 , pp. 9–16, 2019, [Online]. Available: https://sid.ir/paper/263621/en

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