Micropropagation Stevia Medicinal Plant (Stevia rebaudiana) Using Proliferation of Shoot Tip

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Zareil Mahsa; DEZHSETAN SARA; BEHNAMIAN MAHDI

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: GENETIC ENGINEERING AND BIOSAFETY JOURNAL Year:2018 | Volume:6 | Issue:2 Page(s): 245-256

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

561

Download:

Cites:

Information Journal Paper

Title

Micropropagation Stevia Medicinal Plant (Stevia rebaudiana) Using Proliferation of Shoot Tip

Author(s)

Zareil Mahsa | DEZHSETAN SARA | BEHNAMIAN MAHDI | Issue Writer Certificate

Keywords

Hardening

Micropropagation

Plant growth regulators

Stevia

Shoot-tip

Abstract

Stevia rebaudiana is a medicinally important plant, free-calorie with sweet test and beneficial for diabetic patient. In order to optimization of the Micropropagation of this plant, three experiments were conducted by factorial experiment based on completely randomized design (CRD) with three replications. Shoot tip explants were cultured on MS media supplemented with Kin (0, 1 & 2 mg/l) + IAA (0, 0. 5, 1 & 1. 5 mg/l) (test 1), Kin (3 & 4 mg/l) with IAA (0. 5, 1 & 1. 5 mg/l) (test 2) and BAP (0, 1, 2, 3 & 4 mg/l) + IBA (0, 0. 5, 1 & 1. 5 mg/l) (test 3). The shoot number increased with increasing concentrations of Kin in media and in second experiment, the highest shoot number was produced in MS medium supplemented with 4 mg/l Kin + 0. 5 mg/l IAA (with an average of 7 shoots per explant). However, the best result of Micropropagation was obtained from MS medium containing 2 mg/l BAP + 1. 5 mg/l IBA in test 3 (ith an average of 9 shoots per explant). Also, increasing BAP concentrations from 2 mg/l resulted to a significant reduction in shoot number and shoot length and quality of plantlets as well as increase in callusing. In addition, shoot number was dramatically increased by incubation the cultured explants in culture room for two months. The maximum rooting of developed shootlets was achieved from MS medium supplemented with1 mg/l IBA. The rooted plantlets were hardened in culture room and with high successfully established in soil.

Cites

No record.

References

No record.

Cite

APA: Copy

Zareil, Mahsa, DEZHSETAN, SARA, & BEHNAMIAN, MAHDI. (2018). Micropropagation Stevia Medicinal Plant (Stevia rebaudiana) Using Proliferation of Shoot Tip. GENETIC ENGINEERING AND BIOSAFETY JOURNAL, 6(2 ), 245-256. SID. https://sid.ir/paper/270535/en

Vancouver: Copy

Zareil Mahsa, DEZHSETAN SARA, BEHNAMIAN MAHDI. Micropropagation Stevia Medicinal Plant (Stevia rebaudiana) Using Proliferation of Shoot Tip. GENETIC ENGINEERING AND BIOSAFETY JOURNAL[Internet]. 2018;6(2 ):245-256. Available from: https://sid.ir/paper/270535/en

IEEE: Copy

Mahsa Zareil, SARA DEZHSETAN, and MAHDI BEHNAMIAN, “Micropropagation Stevia Medicinal Plant (Stevia rebaudiana) Using Proliferation of Shoot Tip,” GENETIC ENGINEERING AND BIOSAFETY JOURNAL, vol. 6, no. 2 , pp. 245–256, 2018, [Online]. Available: https://sid.ir/paper/270535/en

Related Journal Papers